Structures of a unique O-polysaccharide of Edwardsiella tarda PCM 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing O-polysaccharide shared by E. tarda PCM 1145, PCM 1151 and PCM 1158
| Autor: | Philip V. Toukach, Ewa Katzenellenbogen, Andrzej Gamian, Nina A. Kocharova, Yuriy A. Knirel, Sabina Górska, Maria Bogulska |
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| Rok vydání: | 2012 |
| Předmět: |
Diol
Polysaccharide Biochemistry Analytical Chemistry Propanolamines chemistry.chemical_compound Species Specificity Carbohydrate Conformation Tetrasaccharide skin and connective tissue diseases Edwardsiella tarda Nuclear Magnetic Resonance Biomolecular Hexoses chemistry.chemical_classification Chromatography biology Hexuronic Acids Hydrolysis Organic Chemistry O Antigens General Medicine biology.organism_classification Amides Citrobacter freundii chemistry Propylene Glycols Salmonella enterica Acid hydrolysis Heteronuclear single quantum coherence spectroscopy |
| Zdroj: | Carbohydrate Research. 355:56-62 |
| ISSN: | 0008-6215 |
| Popis: | Lipopolysaccharides of four strains of Edwardsiella tarda were degraded by mild acid hydrolysis, and the released O-polysaccharides were isolated by GPC and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H, (1)H COSY, TOCSY, ROESY, (1)H, (13)C HMBC, HSQC and HSQC-TOCSY experiments. The O-polysaccharide from E. tarda PCM 1153 was found to contain D-GalA, D-GlcNAc, D-Gal and 2-amino-1,3-propanediol (GroN). In the tetrasaccharide repeating unit, GroN is amide-linked to one of the GalA residues, and Gal is non-stoichiometrically 2- or 3-O-acetylated (~45% at each position): [structure: see text]. Three other E. tarda strains examined (PCM 1145, PCM 1151 and PCM 1158) share the following O-polysaccharide structure: [structure: see text] where Abe indicates 3,6-dideoxy-D-xylo-hexose (abequose). This structure resembles those of Citrobacter freundii O22 (PCM 1555) and Salmonella enterica O4. In accordance with the structural data, SDS-PAGE and immunoblotting of the lipopolysaccharides with anti-C. freundii O22 serum demonstrated that the O-antigens of the three E. tarda strains are serologically identical to each other and to the O-antigens of C. freundii O22 and S. enterica O4. |
| Databáze: | OpenAIRE |
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