Astragaloside VI and cycloastragenol-6-O-beta-D-glucoside promote wound healing in vitro and in vivo

Autor: Wen-Liang Chang, Tsu-Chung Chang, Nicholas S. Kirkby, Shih-Yu Lee, Wei-Cheng Tsai, Ching-Huei Ou, Shu-Fen Huang, Zhi-Xiang Li
Rok vydání: 2016
Předmět:
0301 basic medicine
MAPK/ERK pathway
Keratinocytes
Male
Angiogenesis
CHEMICAL-CONSTITUENTS
DIABETIC-RATS
Drug Evaluation
Preclinical
Pharmaceutical Science
Chemistry
Medicinal
Integrative & Complementary Medicine
Pharmacology
ANGIOGENESIS
PATHWAY
chemistry.chemical_compound
0302 clinical medicine
Astragaloside
Glucosides
Drug Discovery
Pharmacology & Pharmacy
Extracellular Signal-Regulated MAP Kinases
Skin
integumentary system
1104 Complementary And Alternative Medicine
ErbB Receptors
ERK
030220 oncology & carcinogenesis
Molecular Medicine
Life Sciences & Biomedicine
RADIX-ASTRAGALI
MAP Kinase Signaling System
EGFR
Medicinal & Biomolecular Chemistry
0607 Plant Biology
Astragaloside VI
Cell Line
Dermal fibroblast
03 medical and health sciences
Cycloastragenol-6-O-beta-D glucoside
In vivo
TELOMERASE
Animals
Humans
Cycloastragenol
Cell Proliferation
REPAIR
Wound Healing
Science & Technology
Plant Sciences
Astragalus Plant
Fibroblasts
Saponins
Triterpenes
Mice
Inbred C57BL
HaCaT
030104 developmental biology
IV
Complementary and alternative medicine
chemistry
1115 Pharmacology And Pharmaceutical Sciences
Receptor
Epidermal Growth Factor
GROWTH-FACTOR RECEPTOR
Wound healing
MEMBRANACEUS BUNGE
Zdroj: Phytomedicine : international journal of phytotherapy and phytopharmacology. 38
ISSN: 1618-095X
Popis: Background Astragalus genus includes most of the common, historical herbal medicines that have various applications in Asian countries. However, clinical data and mechanistic insights into their actions are still lacking. Purpose In this study, we aimed to examine the effects of astragalosides on wound healing in vitro and in vivo, as well as the underlying mechanisms of these actions. Methods The wound healing activity of astragalosides was investigated in human HaCaT keratinocytes, human dermal fibroblast (HDF) cells, and murine models of wound healing. Results All eight astragalosides studied enhanced epidermal growth factor receptor (EGFR) activity in HaCaT cells. Among them, astragaloside VI (AS-VI) showed the strongest EGFR activation. Consistently, AS-VI and cycloastragenol-6-O-beta-D-glucoside (CMG), which is the major metabolite of astragalosides, enhanced extracellular signal-regulated kinase (ERK) activity in a concentration-dependent manner. In agreement, both compounds induced EGFR-dependent cell proliferation and migration in HaCaT and HDF cells. In addition, we showed that AS-VI and CMG accelerated the healing of both sterile and infected wounds in vivo. These effects were associated with increased angiogenesis in the scar tissue. Conclusion AS-VI and CMG increased the proliferation and migration of skin cells via activation of the EGFR/ERK signalling pathway, resulting in the improvement of wound healing in vitro and in vivo. These findings indicate the therapeutic potential of AS-VI and CMG to accelerate wound healing; additionally, they suggest the mechanistic basis of this activity.
Databáze: OpenAIRE
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