Can Extracorporeal Shockwave Promote Osteogenesis of Equine Bone Marrow-Derived Mesenchymal Stem Cells In Vitro?
| Autor: | John D. Kisiday, Jennifer N Phillips, Aimee C. Colbath, Laurie R. Goodrich |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Osteocalcin Bone Morphogenetic Protein 2 Bone Marrow Cells Core Binding Factor Alpha 1 Subunit Biology Extracorporeal High-Energy Shock Waves 03 medical and health sciences 0302 clinical medicine Osteogenesis medicine Animals Osteonectin Horses Cells Cultured Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Hematology Alkaline Phosphatase In vitro Collagen Type III 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Cancer research Bone marrow 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells and Development. 29:110-118 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2019.0202 |
| Popis: | Both bone marrow-derived mesenchymal stem cells (BMDMSCs) and extracorporeal shockwave (ESW) have shown promise for enhancing fracture repair. If exposure of BMDMSCs to ESW enhances osteogenic differentiation, these therapies may be combined in vivo or used as a method for preconditioning BMDMSCs. The objective of this study was to determine the effect of ESW on the osteogenic ability of equine BMDMSCs. We hypothesized that ESW would promote osteogenesis evidenced by increased gene expression, alkaline phosphatase (ALPL) expression, slide morphologic score, and protein expression. BMDMSCs were evaluated from six horses. BMDMSCs were culture expanded to passage 3, dissociated, then placed in conical tubes. Treatment cells ("shocked") were exposed to 500 pulses at 0.16 mJ/mm |
| Databáze: | OpenAIRE |
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