BAC-based sequencing of behaviorally-relevant genes in the prairie vole
| Autor: | Nisc Comparative Sequencing Program, Lisa A. McGraw, Pamela J. Thomas, James W. Thomas, Jamie K. Davis, Larry J. Young |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Candidate gene
Chromosomes Artificial Bacterial Sequence analysis lcsh:Medicine Locus (genetics) Biology Polymorphism Single Nucleotide Nucleotide diversity 03 medical and health sciences 0302 clinical medicine Segmental Duplications Genomic Model Organisms Gene Frequency INDEL Mutation Genetics Animals Amino Acid Sequence lcsh:Science Gene Phylogeny 030304 developmental biology Segmental duplication Animal Management 0303 health sciences Evolutionary Biology Multidisciplinary Behavior Animal Arvicolinae Haplotype lcsh:R Genetic Variation Computational Biology Agriculture Sequence Analysis DNA Genomics biology.organism_classification Prairie vole Genes Veterinary Science lcsh:Q Sequence Alignment Zoology 030217 neurology & neurosurgery Research Article Neuroscience |
| Zdroj: | PLoS ONE, Vol 7, Iss 1, p e29345 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The prairie vole (Microtus ochrogaster) is an important model organism for the study of social behavior, yet our ability to correlate genes and behavior in this species has been limited due to a lack of genetic and genomic resources. Here we report the BAC-based targeted sequencing of behaviorally-relevant genes and flanking regions in the prairie vole. A total of 6.4 Mb of non-redundant or haplotype-specific sequence assemblies were generated that span the partial or complete sequence of 21 behaviorally-relevant genes as well as an additional 55 flanking genes. Estimates of nucleotide diversity from 13 loci based on alignments of 1.7 Mb of haplotype-specific assemblies revealed an average pair-wise heterozygosity (8.4×10(-3)). Comparative analyses of the prairie vole proteins encoded by the behaviorally-relevant genes identified >100 substitutions specific to the prairie vole lineage. Finally, our sequencing data indicate that a duplication of the prairie vole AVPR1A locus likely originated from a recent segmental duplication spanning a minimum of 105 kb. In summary, the results of our study provide the genomic resources necessary for the molecular and genetic characterization of a high-priority set of candidate genes for regulating social behavior in the prairie vole. |
| Databáze: | OpenAIRE |
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