A Lysin motif (LysM)-containing protein functions in antibacterial responses of red swamp crayfish, Procambarus clarkii
| Autor: | Xiao-Fan Zhao, Xiu-Zhen Shi, Jing Zhou, Yu-Ping Jia, Jin-Xing Wang, Jiang-Feng Lan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gills
Vibrio anguillarum Staphylococcus aureus Hemocytes Amino Acid Motifs Immunology Lysin Gene Expression Chitin Astacoidea Aquatic Science Arthropod Proteins Microbiology Bacterial Proteins Complementary DNA Environmental Chemistry Animals Gene Phylogeny Vibrio Procambarus clarkii Innate immune system biology General Medicine Sequence Analysis DNA biology.organism_classification Up-Regulation Open reading frame Biochemistry Organ Specificity Host-Pathogen Interactions Proteoglycans Peptidoglycan binding Bacteria Antimicrobial Cationic Peptides Bacillus subtilis Protein Binding Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 40:311-319 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2013.03.011 |
| Popis: | Lysin domain (LysM) is a widely spread domain in nature and could bind different peptidoglycans and chitin-like compounds in bacteria and eukaryotes. In plants, Lysin motif containing proteins are one of the major classes of pattern recognition proteins which can recognize GlcNAc-containing glycans and have important functions in plant immunity. However, their functions in animal immunity are still unclear. In this study, a cDNA encoding a LysM containing protein was identified from red swamp crayfish, Procambarus clarkii. The cDNA of PcLysM contained 1200 base pair nucleotides with an open reading frame of 702 bp encoding a protein of 233 amino acid residues. The deduced protein had a calculated molecular mass of 25.950 kDa and a pI of 6.84. Tissue distribution analysis in mRNA level showed that it was highly expressed in gills, hemocytes, and intestine, and lowly expressed in hearts, hepatopancreas, and stomach. Time course expression pattern analysis showed that PcLysM was upregulated in hemocytes and gills after challenge with Vibrio anguillarum, and it was upregulated at 12 h after challenge with Staphylococcus aureus in gills. The recombinant PcLysM could bind to different bacteria, and yeast. Further study revealed that PcLysM could bind to peptidoglycans from different bacteria, and chitin. After PcLysM was knocked down, the upregulation of antimicrobial peptide (AMP) genes (crustins and antilipopolysaccharide factors) was suppressed in response to bacterial infection in gills. These results suggest that PcLysM recognizes different microorganisms through binding to polysaccharides, such as peptidoglycans and chitin and regulates the expression of some antimicrobial peptide genes though unknown pathways and regulates the expression of some antimicrobial peptide genes though unknown pathways. This study might provide a clue to elucidate the roles of PcLysM in the innate immune reaction of crayfish P. clarkii. |
| Databáze: | OpenAIRE |
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