Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae
| Autor: | I Van Megen, Willem Pieter Martin Hoekstra, P Pouwels, H Bergmans, Marca H. M. Wauben, Betty E. Enger-Valk, N Riegman, I van Die |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
animal structures
Macromolecular Substances Protein subunit Molecular Sequence Data Fimbria DNA Recombinant Biology Microbiology Epitope Fimbriae Proteins Bacterial Proteins Escherichia coli Amino Acid Sequence Molecular Biology Peptide sequence Genetics chemistry.chemical_classification Base Sequence Structural gene biochemical phenomena metabolism and nutrition bacterial infections and mycoses female genital diseases and pregnancy complications Hypervariable region Amino acid Genes chemistry Genes Bacterial Fimbriae Bacterial bacteria Chromosome Deletion Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 170:5870-5876 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.170.12.5870-5876.1988 |
| Popis: | The influence of genetic manipulation of the structural genes coding for major P-fimbrial subunits on the formation of fimbriae in Escherichia coli was studied. Deletion of two regions that code for hypervariable parts of the P fimbrillin resulted in strong reduction or total absence of fimbria production. Replacement of deleted amino acids by other amino acid residues restored the formation of fimbriae. The hypervariable regions may be important for biogenesis of fimbriae by imposing correct spacing between conserved regions of the protein. The potential for substituting amino acids in the P-fimbrial subunit opens interesting possibilities for use of fimbriae as carriers of foreign antigenic determinants. An antigenic determinant of foot-and-mouth disease virus (FMDV) was incorporated in the F11 fimbrial subunit. Hybrid fimbriae, recognized by an FMDV-specific neutralizing monoclonal antibody directed against FMDV, were formed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|