Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust
| Autor: | Roxana Villca, Jorge Quillaguamán, Felipe Orozco-Gutierrez, Luis Romero-Soto, José Vega-Baudrit, Diego Chambi, Carlos Martín, Rajni Hatti-Kaul, Cristhian Carrasco |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Exopolysaccharides Quinoa stalks quinoa stalks Curupaú sawdust Plant Science Polysaccharide Microbiology 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Hydrolysate Lignocellulose bioconversion lignocellulose bioconversion 03 medical and health sciences chemistry.chemical_compound Halotolerant bacterial isolate 010608 biotechnology Centrifugation Food science Trichloroacetic acid Ethanol precipitation 030304 developmental biology chemistry.chemical_classification 0303 health sciences lcsh:TP500-660 biology Chemistry exopolysaccharides halotolerant bacterial isolate biology.organism_classification lcsh:Fermentation industries. Beverages. Alcohol Salar de Uyuni Mikrobiologi visual_art visual_art.visual_art_medium Halotolerance Sawdust bacterial cultivation Bacterial cultivation Bacteria Food Science |
| Zdroj: | Fermentation, Vol 7, Iss 33, p 33 (2021) Fermentation Volume 7 Issue 1 Pages: 33 |
| ISSN: | 2311-5637 |
| Popis: | The halotolerant bacterial strain BU-4, isolated from a hypersaline environment, was identified as an exopolysaccharide (EPS) producer. Pretreatment liquids of steam-exploded quinoa stalks and enzymatic hydrolysates of Curupaú sawdust were evaluated as carbon sources for EPS production with the BU-4 strain, and the produced EPS was characterized using FTIR, TGA, and SEM. Cultivation was performed at 30 °C for 48 h, and the cells were separated from the culture broth by centrifugation. EPS was isolated from the cell pellets by ethanol precipitation, and purified by trichloroacetic acid treatment, followed by centrifugation, dialysis, and freeze-drying. EPS production from quinoa stalks- and Curupaú sawdust-based substrates was 2.73 and 0.89 g L−1, respectively, while 2.34 g L−1 was produced when cultivation was performed on glucose. FTIR analysis of the EPS revealed signals typical for polysaccharides, as well as ester carbonyl groups and sulfate groups. High thermal stability, water retention capacity and gel-forming ability were inferred from SEM and TGA. The capability of the halotolerant isolate for producing EPS from pretreatment liquids and hydrolysates was demonstrated, and characterization of the EPS revealed their broad application potential. The study shows a way for producing value-added products from waste materials using a bacterium from a unique Bolivian ecosystem. |
| Databáze: | OpenAIRE |
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