Autor: |
K. Ta, F. Sabot, H. Adam, Y. Vigouroux, S. De Mita, A. GhesquièRe, N. Do, P. Gantet, S. Jouannic |
Rok vydání: |
2016 |
DOI: |
10.6084/m9.figshare.c.3612944_d3 |
Popis: |
Sequences and phasiRNA abundances of the 4 phased loci used for validation. The 4 phased loci reported here were used for stem-loop RT-PCR (phasiRNAs) and classical RT-PCR (lncRNAs) validation. The positions of the phased loci on MSU7.0 chromosomes are indicated. The alignment between miR2118f and the phased locus is indicated. The miR2118 recognition site is in blue. The phasiRNAs detected in the plus strand are highlighted alternately in green and in red. The phasiRNAs detected in the minus strand are underlined alternately in green and in red. The phasiRNA boxed in yellow is the most abundant one and the one used for stem-loop RT-PCR validation and qRT-PCR analysis. Histograms illustrate the relative abundance of the detected phasiRNAs per locus in the two species (PDF 159 kb) |
Databáze: |
OpenAIRE |
Externí odkaz: |
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