Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation
| Autor: | Ran Gao, Sheng Yu, Gang Hu, Shusen Zhai, Zhen Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Epidemiology Hepatocellular carcinoma miR-18a Normal tissue Infectious and parasitic diseases RC109-216 law.invention 03 medical and health sciences 0302 clinical medicine law Circular RNA circRHOBTB3 Medicine RC254-282 030304 developmental biology 0303 health sciences Expression vector business.industry Cell growth Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease digestive system diseases Infectious Diseases Oncology 030220 oncology & carcinogenesis Cancer research Precursor Suppressor business Research Article |
| Zdroj: | Infectious Agents and Cancer, Vol 16, Iss 1, Pp 1-7 (2021) Infectious Agents and Cancer |
| ISSN: | 1750-9378 |
| Popis: | Background Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. Methods In this study, circRHOBTB3, mature miR-18a, and miR-18a precursor in HCC and paired non-cancer tissues were detected by RT-qPCR. The role of circRHOBTB3 in the production of mature miR-18a was explored by transfecting circRHOBTB3 expression vector into HCC cells, followed by RT-qPCR to determine the expression of mature miR-18a and miR-18a precursor. The role of circRHOBTB3 and miR-18a in HCC cell proliferation was studied using CCK-8 assay. Results CircRHOBTB3 was under-expressed in HCC compared to normal tissues. In HCC cells, circRHOBTB3 overexpression decreased mature miR-18a level but not miR-18a precursor. Cell proliferation analysis showed that circRHOBTB3 overexpression decreased cell proliferation while miR-18a overexpression increased cell proliferation. Moreover, circRHOBTB3 suppressed the role of miR-18a in cell proliferation. Conclusions CircRHOBTB3 is downregulated in HCC and may suppress cell proliferation by reducing miR-18a production. |
| Databáze: | OpenAIRE |
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