Studying the proliferation of human peripheral blood T lymphocytes in serum-free medium
| Autor: | V. U. Tabakov, J. V. Schepkina, A. A. Jarilin, M. M. Litvina, V. V. Chestkov |
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| Rok vydání: | 2009 |
| Předmět: |
Interleukin 2
medicine.medical_specialty CD3 Complex Lymphocyte CD3 T-Lymphocytes Serum free medium General Biochemistry Genetics and Molecular Biology Culture Media Serum-Free Andrology Internal medicine medicine Humans Phytohemagglutinins Cells Cultured Cell Proliferation biology Chemistry Cell growth General Medicine Peripheral blood Endocrinology medicine.anatomical_structure CD4 Antigens biology.protein Interleukin-3 Fetal bovine serum medicine.drug |
| Zdroj: | Bulletin of experimental biology and medicine. 147(1) |
| ISSN: | 0007-4888 |
| Popis: | We compared the cultivation of human peripheral blood lymphocytes in serum-free medium Hybris-2 and RPMI 1640 medium with 10% fetal bovine serum in the presence of phytohemagglutinin and interleukin-2. The optimal concentration of phytohemagglutinin significantly differed in serum-free and serum-containing media (0.5 and 5 microg/ml, [corrected] respectively). Both mitogens were more potent in stimulating the proliferation of lymphocytes in serum-free medium than in serum-containing medium. Strong proliferation of CD3(+) and CD4(+) T lymphocytes was observed in both media. The dynamics of other markers was similar in serum-free and serum-containing media. However, significant differences were revealed between individual donors. Our results indicate that the developed serum-free medium may be used in lymphocyte cultivation for scientific, diagnostic, and therapeutic purposes. |
| Databáze: | OpenAIRE |
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