The fibrillar organisation of the osteon and cellular aspects of its development : a morphological study using the SEM fractured cortex technique
| Autor: | Guido Zarattini, Marcella Marchese, Ugo E. Pazzaglia, Terenzio Congiu, Daniela Elena Quacci |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Materials science Fibrillar Collagens Matrix (biology) Bone remodelling Cortex (anatomy) medicine Animals Lamellar structure Osteon Fibril organization Osteocyte Osteoblast Osteoclast General Medicine Anatomy Resorption Haversian System Apposition medicine.anatomical_structure Microscopy Electron Scanning Bone Remodeling Rabbits Biomedical engineering |
| Zdroj: | Anatomical science international. 86(3) |
| ISSN: | 1447-073X |
| Popis: | The collagen architecture of secondary osteons was studied with scanning electron microscopy (SEM) employing the fractured cortex technique and osmic maceration. Fibrillar orientation and the change in their direction in sequential lamellae was documented where lamellar formation was ongoing, as well as in resorption pits where osteoclasts had exposed the collagen organisation of the underlying layers. Applying an adaptive stereo matching technique, the mean thickness of matrix layers removed by osteoclasts was 1.36 ± 0.45 μm. It was also documented that osteoclasts do not attack the cellular membrane of the exposed osteocytes. The mean linear osteoblast density in fractured hemicanals was assessed with SEM and no significant differences were observed comparing larger with smaller central canal osteons. These findings suggested a balance between the differentiated osteoblasts that have aligned on the surface of the cutting cone and those that are transformed into osteocytes, because the canal surface is progressively reduced as the lamellar apposition advances. |
| Databáze: | OpenAIRE |
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