The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines
| Autor: | E Anteka, Maria Cristina Martins, Zelia M. Correa, Jc Marshall, Miguel N. Burnier, J P de Souza Filho, Anamaria Baptista Coutinho |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Retinal Neoplasms Blotting Western Nepafenac chemistry.chemical_compound Western blot Tumor Cells Cultured medicine Humans Cell Proliferation Phenylacetates Cyclooxygenase 2 Inhibitors medicine.diagnostic_test Chemistry Cell growth Retinoblastoma medicine.disease Molecular biology Blot Ophthalmology Cyclooxygenase 2 Cell culture COX-2 inhibitor Amfenac medicine.drug |
| Zdroj: | Eye. 20:598-601 |
| ISSN: | 1476-5454 0950-222X |
| Popis: | To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb)cell lines.Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by immunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. The averaged results per condition were recorded. The Student's t-test was used to compare results from the cells cultured with and without amfenac.The Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. The two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immunocytochemical analysis.The selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb. |
| Databáze: | OpenAIRE |
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