Serotype-associated polymorphisms in a partial rpob gene sequence of salmonella enterica
| Autor: | Marlen Barreto, Pedro Abalos, Patricio Retamal, Marcela Fresno, Sindy Gutierrez, Catherine Dougnac |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Serotype Immunology Subspecies Applied Microbiology and Biotechnology Microbiology Bacterial Proteins Genetics Animals Humans Serotyping Molecular Biology Gene Phylogeny Sequence (medicine) Polymorphism Genetic Base Sequence biology Strain (biology) Salmonella enterica General Medicine RNA-Dependent RNA Polymerase biology.organism_classification rpoB Sequence Alignment Bacteria Multilocus Sequence Typing |
| Zdroj: | CANADIAN JOURNAL OF MICROBIOLOGY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Salmonella enterica is a zoonotic bacterium with more than 2500 serotypes, which affect a wide range of hosts and produce diverse clinical outcomes. Strain identification usually involves costly and time-demanding procedures. This paper describes the sequencing of a rpoB hypervariable gene segment (847 bp) that allows identification of serotypes in S. enterica strains isolated from several hosts. The nucleotide similarity values among S. enterica serotypes ranged from 98.23% to 99.88%, with potential usefulness for devising a simple one-step sequencing as a first approach for identification of S. enterica strains. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can discriminate S. enterica strains at the subspecies level. |
| Databáze: | OpenAIRE |
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