Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8
| Autor: | Yusuke Yamada, Koichiro Shimomura, Yoshihiro Okabe, Hiroshi Ezura, Mina Katayama, Kaori Yoneyama, Junna Saito, Shoko Hasegawa, Kohki Akiyama, Mikihisa Umehara, Shinjiro Yamaguchi, Koh Aoki, Masato Shindo, Takuya Tsutsumi, Shunsuke Fukushima, Tohru Ariizumi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine TILLING Mutant strigolactones Biology 01 natural sciences Catalysis lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences Solanum lycopersicum shoot branching Cultivar Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Phelipanche aegyptiaca Organic Chemistry Rhizotron fungi food and beverages carotenoid cleavage dioxygenase 8 General Medicine biology.organism_classification Orobanche minor plant_sciences Computer Science Applications Horticulture 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Germination Shoot Rootstock 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 19 Issue 9 International Journal of Molecular Sciences, Vol 19, Iss 9, p 2645 (2018) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19092645 |
| Popis: | Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa&mdash hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the &ldquo targeting induced local lesions in genomes&rdquo (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca. |
| Databáze: | OpenAIRE |
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