Heterogeneous Nuclear Ribonucleoprotein H1, a Novel Nuclear Autoantigen
| Autor: | Herbert Hooijkaas, Diana Dufour, Benoit Devogelaere, Etienne Waelkens, Jörg Klug, Norbert Blanckaert, Xavier Bossuyt, Patrick Verschueren, Monika Fijak, Karolien Van den Bergh, Georges Michiels, Rita Derua, Joop P. van de Merwe, Daniel Engelbert Blockmans, Humbert de Smedt, Rene Westhovens, Katrijn Op De Beéck |
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| Přispěvatelé: | Public Health, Immunology |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Anti-nuclear antibody Extractable nuclear antigens Blotting Western Clinical Biochemistry Immunofluorescence Cohort Studies Young Adult Antigen medicine Animals Humans Fluorescent Antibody Technique Indirect Aged Autoantibodies Ribonucleoprotein Aged 80 and over Heterogeneous-Nuclear Ribonucleoprotein Group F-H biology medicine.diagnostic_test Biochemistry (medical) Autoantibody Middle Aged medicine.disease Connective tissue disease Recombinant Proteins Rats stomatognathic diseases Sjogren's Syndrome ROC Curve Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology biology.protein Female Antibody |
| Zdroj: | Clinical Chemistry, 55(5), 946-954. American Association for Clinical Chemistry Inc. |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Background: Serum samples from patients with autoimmune connective tissue diseases that show a finely speckled antinuclear antibody (ANA) on indirect immune-fluorescence often have antibodies against unknown nuclear target antigens. To search for such autoantigens we applied a proteomic approach using sera from patients with a high ANA titer (≥640) and finely speckled fluorescence but in whom no antibodies to extractable nuclear antigens (ENA) could be identified.Methods: Using an immunoproteomics approach we identified heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1) as a novel nuclear target of autoantibody response.Results: Recombinant rat hnRNP H1 reacted in Western blot analyses with 48% of 93 sera from patients with primary Sjögren syndrome and with 5.2% of 153 sera from patients with other connective tissue diseases (diseased controls). For comparison, the diagnostic sensitivity and specificity of anti–Sjögren syndrome A (SSA) antibodies for primary Sjögren syndrome in the same patient cohort were 88.2% and 76.3%, respectively. Interestingly, 5 of 11 primary Sjögren syndrome patients with no anti-SSA or anti-SSB antibodies had anti–hnRNP H1 antibodies. Anti–hnRNP H1 antibodies were preabsorbed by hnRNP H1, as demonstrated by indirect immunofluorescence. In an evaluation of the presence of anti–hnRNP H1 antibodies in 188 consecutive samples submitted to the clinical laboratory with positive ANA (titer ≥160), anti–hnRNP H1 antibodies were found in 3 of 7 (2 primary and 5 secondary) Sjögren syndrome patients and in 8.3% of the diseased controls.Conclusions: HnRNP H1 is a newly discovered autoantigen that could become an additional diagnostic marker. |
| Databáze: | OpenAIRE |
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