Modulation of nitric oxide and cytokines production by l-arginine in human gut mucosa
| Autor: | Philippe Ducrotté, André Petit, Jonathan Leblond, Sabrina Lemoulan, Pierre Déchelotte, Moïse Coëffier, Aurelie Hubert, R. Marion, Stéphane Lecleire |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
medicine.medical_specialty Arginine Duodenum Biopsy medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Inflammation Nitric Oxide Critical Care and Intensive Care Medicine Enteral administration Statistics Nonparametric Nitric oxide Basal (phylogenetics) chemistry.chemical_compound Enteral Nutrition Griess test Internal medicine medicine Humans RNA Messenger Intestinal Mucosa Cross-Over Studies Nutrition and Dietetics medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Interleukins Inflammatory Bowel Diseases Endocrinology Cytokine chemistry medicine.symptom business |
| Zdroj: | Clinical Nutrition. 24:353-359 |
| ISSN: | 0261-5614 |
| DOI: | 10.1016/j.clnu.2004.11.011 |
| Popis: | Summary Background : Arginine is a conditionally essential amino-acid with immuno-modulatory properties, mainly through the nitric oxide (NO) pathway. Aim : To assess the effects of arginine on intestinal production of pro- and anti-inflammatory cytokines and NO in human gut. Methods : An enteral solution of arginine or a control solution of amino-acids was administered to 8 healthy volunteers on a randomized cross-over design. Duodenal biopsies were taken. Pro- (IL-6, IL-8) and anti-inflammatory (IL-4, IL-10) cytokines mRNA expression was assessed by RT-PCR. Other biopsies were cultured with 0.1, 0.5 or 2mM arginine or control amino-acids, under basal or IL-1 β -induced inflammatory conditions. Interleukin-4, IL-6, IL-8 and IL-10 production was measured in culture supernatant by ELISA and NO production by Griess reaction. Results : Arginine enhanced the production of NO under inflammatory conditions in a dose-dependent manner ( P = 0.03 ). IL-1 β increased the production of IL-8 and IL-6 ( P 0.01 ). Arginine had no effect on pro- and anti-inflammatory cytokines production both under basal and inflammatory conditions. Conclusions : Arginine enhanced the production of NO but did not affect that of cytokines in inflammatory human gut. Further clinical studies are required to assess whether arginine-enhanced NO production plays a beneficial or deleterious effect in intestinal inflammation. |
| Databáze: | OpenAIRE |
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