Expression of caspase activation recruitment and pyrin domain levels of apoptosis-associated speck-like protein complex in the pancreas of rats subjected to experimental pancreatitis
| Autor: | Periyanayagam Suguna, Ravikumar Aruna, Geetha A |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Rutin Health Toxicology and Mutagenesis Caspase 1 Inflammation Toxicology Pyrin domain chemistry.chemical_compound Downregulation and upregulation Internal medicine medicine Animals RNA Messenger Rats Wistar Dose-Response Relationship Drug Ethanol biology Chemistry General Medicine Dietary Fats Rats CARD Signaling Adaptor Proteins Endocrinology Gene Expression Regulation Pancreatitis Apoptosis Myeloperoxidase Immunology biology.protein Cytokines Interleukin 18 medicine.symptom Apoptosis Regulatory Proteins |
| Zdroj: | Human & Experimental Toxicology. 33:940-948 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | The present study investigated the effect of rutin, a natural flavonoid, on the expression of caspase activation recruitment domain (CARD) and pyrin domain (PYD) of apoptosis-associated speck-like protein (ASC), a mediator of inflammation, in the pancreas of rats administered with ethanol (EtOH) and high-fat diet (HFD). Pancreatitis was induced in male albino Wistar rats by administering EtOH (8–12 g/kg/day) and HFD (22% fat) for 90 days. In addition, rats also received 100 mg rutin/kg body weight orally from 31st day till the experimental period. Serum levels of cytokines, interleukin 18 (IL-18) and IL-6; activity levels of caspase-1 and myeloperoxidase (MPO); messenger RNA (mRNA) expression of tumor necrosis factor α (TNF-α), caspase-1, CARD and PYD of ASC; and histological changes in pancreas were assessed. We observed a significant elevation in serum IL-18, IL-6, caspase-1 and MPO activities, mRNA expression of PYD, TNF-α and caspase-1 in the pancreas of rats administered with EtOH and HFD. Rutin administration along with EtOH and HFD significantly upregulated the mRNA expression of CARD and downregulated PYD, caspase-1, and TNF-α expressions. Rutin supplementation was also found to reduce IL-18 and IL-6 levels; and inflammatory changes in tissue architecture were evidenced by histological observations. The anti-inflammatory activity of rutin might be due to its effect on modulating the expression of ASC complex that mediates inflammation. |
| Databáze: | OpenAIRE |
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