Pegylated and nanoparticle-conjugated sulfonium salt photo triggers necrotic cell death
| Autor: | Ebrahim H. Ghazvini Zadeh, Mykhailo V. Bondar, Alaa Abbas Fadhel, Xiling Yue, Kevin D. Belfield |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Light
medicine.medical_treatment Pharmaceutical Science Photodynamic therapy silica nanoparticles 01 natural sciences Polyethylene Glycols chemistry.chemical_compound International Journal of Nanomedicine Drug Discovery Original Research Photosensitizing Agents Cell Death Singlet Oxygen Singlet oxygen General Medicine Silicon Dioxide 3. Good health Biochemistry photoacid generator Hydrophobic and Hydrophilic Interactions Programmed cell death Materials science Endosome Biophysics Sulfonium Compounds Bioengineering Polyethylene glycol Conjugated system 010402 general chemistry stimuli-responsive Biomaterials Necrosis medicine Cell Adhesion Humans Cell adhesion oxygen-independent photodynamic therapy Photons 010405 organic chemistry Organic Chemistry sulfonium salt HCT116 Cells 0104 chemical sciences Oxygen chemistry Photochemotherapy 13. Climate action Cancer cell Nanoparticles |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Alaa A Fadhel,1 Xiling Yue,1 Ebrahim H Ghazvini Zadeh,1 Mykhailo V Bondar,2 Kevin D Belfield3 1Department of Chemistry, University of Central Florida, Orlando, FL, USA; 2Institute of Physics NASU, Kiev, Ukraine; 3Department of Chemistry and Environmental Science, College of Science and Liberal Arts, New Jersey Institute of Technology, University Heights, Newark, NJ, USA Abstract: Photodynamic therapy (PDT) processes involving the production of singlet oxygen face the issue of oxygen concentration dependency. Despite high oxygen delivery, a variety of properties related to metabolism and vascular morphology in cancer cells result in hypoxic environments, resulting in limited effectiveness of such therapies. An alternative oxygen-independent agent whose cell cytotoxicity can be remotely controlled by light may allow access to treatment of hypoxic tumors. Toward that end, we developed and tested both polyethylene glycol (PEG)-functionalized and hydrophilic silica nanoparticle (SiNP)-enriched photoacid generator (PAG) as a nontraditional PDT agent to effectively induce necrotic cell death in HCT-116 cells. Already known for applications in lithography and cationic polymerization, our developed oxygen-independent PDT, whether free or highly monodispersed on SiNPs, generates acid when a one-photon (1P) or two-photon (2P) excitation source is used, thus potentially permitting deep tissue treatment. Our study shows that when conjugated to SiNPs with protruding amine functionalities (SiNP–PAG9), such atypical PDT agents can be effectively delivered into HCT-116 cells and compartmentalize exclusively in lysosomes and endosomes. Loss of cell adhesion and cell swelling are detected when an excitation source is applied, suggesting that SiNP–PAG9, when excited via near-infrared 2P absorption (a subject of future investigation), can be used as a delivery system to selectively induce cell death in oxygen-deprived optically thick tissue. Keywords: oxygen-independent photodynamic therapy, photoacid generator, silica nanoparticles, stimuli-responsive, sulfonium salt |
| Databáze: | OpenAIRE |
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