A multiplex assay for the simultaneous detection of antibodies against 15 Plasmodium falciparum and Anopheles gambiae saliva antigens

Autor: Karine Puget, Eve Orlandi-Pradines, Adama Tall, Jean Biram Sarr, Chloé Dumoulin, Aissatou Toure-Balde, Franck Remoue, Cheikh Sokhna, Thierry Fusai, Jean-François Trape, Anne Poinsignon, Aurélie Pascual, Elena Ambrosino, Pierre Druilhe, Christophe Rogier
Přispěvatelé: Sociale Geneeskunde, RS: CAPHRI School for Public Health and Primary Care, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Time Factors
Anopheles gambiae
0302 clinical medicine
Multiplex
MESH: Animals
MESH: Plasmodium falciparum
Immunoassay
0303 health sciences
biology
Senegal
3. Good health
Europe
MESH: Reproducibility of Results
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Immunoassay
Adult
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
Plasmodium falciparum
030231 tropical medicine
MESH: Malaria
MESH: Parasitology
Sensitivity and Specificity
Antibodies
lcsh:Infectious and parasitic diseases
MESH: Anopheles
03 medical and health sciences
Immune system
Antigen
MESH: Insect Bites and Stings
MESH: Senegal
Anopheles
parasitic diseases
medicine
Animals
Humans
lcsh:RC109-216
MESH: Saliva
Saliva
030304 developmental biology
MESH: Humans
Research
MESH: Antibodies
MESH: Time Factors
Insect Bites and Stings
Reproducibility of Results
MESH: Adult
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
medicine.disease
biology.organism_classification
Virology
MESH: Sensitivity and Specificity
Malaria
Parasitology
Vector (epidemiology)
Immunology
MESH: Europe
Zdroj: Malaria Journal, 9. BioMed Central Ltd
Malaria Journal
Malaria Journal, BioMed Central, 2010, 9 (1), pp.317. ⟨10.1186/1475-2875-9-317⟩
Malaria Journal, Vol 9, Iss 1, p 317 (2010)
Malaria Journal, 2010, 9 (1), pp.317. ⟨10.1186/1475-2875-9-317⟩
ISSN: 1475-2875
DOI: 10.1186/1475-2875-9-317⟩
Popis: Background Assessment exposure and immunity to malaria is an important step in the fight against the disease. Increased malaria infection in non-immune travellers under anti-malarial chemoprophylaxis, as well as the implementation of malaria elimination programmes in endemic countries, raises new issues that pertain to these processes. Notably, monitoring malaria immunity has become more difficult in individuals showing low antibody (Ab) responses or taking medications against the Plasmodium falciparum blood stages. Commonly available techniques in malaria seroepidemiology have limited sensitivity, both against pre-erythrocytic, as against blood stages of the parasite. Thus, the aim of this study was to develop a sensitive tool to assess the exposure to malaria or to bites from the vector Anopheles gambiae, despite anti-malarial prophylactic treatment. Methods Ab responses to 13 pre-erythrocytic P. falciparum-specific peptides derived from the proteins Lsa1, Lsa3, Glurp, Salsa, Trap, Starp, CSP and Pf11.1, and to 2 peptides specific for the Anopheles gambiae saliva protein gSG6 were tested. In this study, 253 individuals from three Senegalese areas with different transmission intensities and 124 European travellers exposed to malaria during a short period of time were included. Results The multiplex assay was optimized for most but not all of the antigens. It was rapid, reproducible and required a small volume of serum. Proportions of Ab-positive individuals, Ab levels and the mean number of antigens (Ags) recognized by each individual increased significantly with increases in the level of malaria exposure. Conclusion The multiplex assay developed here provides a useful tool to evaluate immune responses to multiple Ags in large populations, even when only small amounts of serum are available, or Ab titres are low, as in case of travellers. Finally, the relationship of Ab responses with malaria endemicity levels provides a way to monitor exposure in differentially exposed autochthonous individuals from various endemicity areas, as well as in travellers who are not immune, thus indirectly assessing the parasite transmission and malaria risk in the new eradication era.
Databáze: OpenAIRE
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