Electrical Detection of Single Methylcytosines in a DNA Oligomer
| Autor: | Masateru Taniguchi, Masayuki Furuhashi, Makusu Tsutsui, Tomoji Kawai, Takahito Ohshiro, Kazuki Matsubara |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Analytical chemistry
Methylation Biochemistry Oligomer Catalysis Nucleobase Cytosine chemistry.chemical_compound symbols.namesake Colloid and Surface Chemistry Electrochemistry A-DNA Electrodes HOMO/LUMO Oligonucleotide Chemistry Fermi level Electric Conductivity DNA Deoxycytidine Monophosphate General Chemistry Crystallography Oligodeoxyribonucleotides Electrode symbols Gold |
| Zdroj: | Journal of the American Chemical Society. 133:9124-9128 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja203839e |
| Popis: | We report label-free electrical detections of chemically modified nucleobases in a DNA using a nucleotide-sized electrode gap. We found that methyl substitution contributes to increase the tunneling conductance of deoxycytidines, which was attributed to a shift of the highest occupied molecular orbital level closer to the electrode Fermi level by methylation. We also demonstrate statistical identifications of methylcytosines in an oligonucleotide by tunneling current. This result suggests a possible use of the transverse electron-transport method for a methylation level analysis. |
| Databáze: | OpenAIRE |
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