Candida krusei form mycelia along agar surfaces towards each other and other Candida species
| Autor: | Corey D. Broeckling, S. Lyons, Jacob Fleischmann |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Indoles food.ingredient 030106 microbiology Candida glabrata Medical and Health Sciences Microbiology 03 medical and health sciences chemistry.chemical_compound food Candida krusei Candida albicans Tryptophol Agar Mycelium Candida Agricultural and Veterinary Sciences biology Farnesol Biological Sciences Phenylethyl Alcohol biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Yeast Culture Media stomatognathic diseases chemistry Microbial Interactions Research Article |
| Zdroj: | BMC Microbiology BMC microbiology, vol 17, iss 1 |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-017-0972-z |
| Popis: | Background Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiments to study this interaction. Results When C.krusei yeast cells are plated in parallel streaks, they form mycelia along agar surfaces toward other yeasts. They also detect the presence of Candida albicans and Candida glabrata across agar surfaces, while the latter two react neither to their own kind, nor to C. krusei. Secreted molecule(s) are likely involved as C.krusei does not react to heat killed C. krusei. Timing and rate of mycelia formation across distances suggests that mycelia start forming when a secreted molecule(s) on agar surface reaches a certain concentration. We detected farnesol, tyrosol and tryptophol molecules that may be involved with mycelial formation, on the agar surfaces between yeast streaks. Unexpectedly the amounts detected between streaks were significantly higher than would have expected from additive amounts of two streaks. All three Candida species secreted these molecules. When tested on agar surface however, none of these molecules individually or combined induced mycelia formation by C. krusei. Conclusions Our data confirms another communal interaction by C. krusei, manifested by formation of mycelia by yeast cells toward their own kind and other yeasts on agar surfaces. We detected secretion of farnesol, tyrosol and tryptophol by C. krusei but none of these molecules induced this activity on agar surface making it unlikely that they are the ones utilized by this yeast for this activity. |
| Databáze: | OpenAIRE |
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