DETECTION OF ANTI-SP100 ANTIBODIES IN PRIMARY BILIARY CIRRHOSIS. COMPARISON OF ELISA AND IMMUNOFLUORESCENCE
| Autor: | Alicja Bauer, Ewa Kraszewska, Andrzej Habior |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Anti-nuclear antibody Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Immunofluorescence Autoantigens Sensitivity and Specificity Primary biliary cirrhosis Antigen Predictive Value of Tests Humans Immunology and Allergy Medicine Fluorescent Antibody Technique Indirect Aged Autoantibodies medicine.diagnostic_test biology Liver Cirrhosis Biliary business.industry Autoantibody Antigens Nuclear IIf Middle Aged medicine.disease Mitochondria Medical Laboratory Technology Antibodies Antinuclear Predictive value of tests biology.protein Female Antibody business |
| Zdroj: | Journal of Immunoassay and Immunochemistry. 34:346-355 |
| ISSN: | 1532-4230 1532-1819 |
| DOI: | 10.1080/15321819.2012.741088 |
| Popis: | Primary biliary cirrhosis (PBC) is, which a chronic, autoimmune liver disease. Some patients have antinuclear antibodies anti-Sp100, which are considered to be disease-specific. We compared an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF) for detection of anti-Sp100. The sensitivity of anti-Sp100 determined by ELISA and IIF was 44% and 34%, respectively. Specificity was 99% for ELISA and 98% for IIF, respectively. The positive and negative predictive value (PPV, NPV) for anti-Sp100 determined by ELISA were 98%, 60% and 95%, 56% for IIF respectively. IIF required substantial experience in interpreting subjective patterns, whereas ELISA was more sensitive, cheaper, less time consuming, and produced clear-cut quantitative results. |
| Databáze: | OpenAIRE |
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