Synthesis and properties of purine-type base-discriminating fluorescent (BDF) nucleosides: distinction of thymine by fluorescence-labeled deoxyadenosine derivatives

Autor:	Kaori Motegi, Kazuo Hanawa, Yoshio Saito, Akimitsu Okamoto, Kenji Omoto, Isao Saito
Rok vydání:	2005
Předmět:	Purine Organic Chemistry Biochemistry Combinatorial chemistry Adenosine Fluorescence Thymine chemistry.chemical_compound chemistry Drug Discovery medicine Deoxyadenosine Derivatives Nucleoside Derivative (chemistry) DNA medicine.drug
Zdroj:	Tetrahedron Letters. 46:7605-7608
ISSN:	0040-4039
DOI:	10.1016/j.tetlet.2005.08.157
Popis:	Novel base-discriminating fluorescent (BDF) nucleoside, 8-fluorescence-labeled adenosine derivative ( 8Py A ), was developed for the detection of thymine base on a target DNA. The BDF nucleoside was incorporated into oligodeoxynucleotides by post-synthetic modification. BDF probes containing 8Py A selectively emit fluorescence only when the base opposite BDF nucleoside is thymine and act as effective reporter probes for homogeneous SNP typing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6565e2318f36d59afda924c1b14a7952 https://doi.org/10.1016/j.tetlet.2005.08.157 Zobrazit plný text záznamu Full Text from ScienceDirect