Stereo images of vesicular stomatitis virus assembly
| Autor: | Monique Dubois-Dalcq, Heinz Arnheiter, Robert A. Lazzarini, W F Odenwald |
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| Jazyk: | angličtina |
| Rok vydání: | 1986 |
| Předmět: |
Genes
Viral G protein Viral protein Immunoelectron microscopy viruses Immunology Matrix (biology) Biology medicine.disease_cause Kidney Microbiology Vesicular stomatitis Indiana virus Viral Matrix Proteins Viral Proteins Capsid Viral Envelope Proteins Cricetinae Virology Baby hamster kidney cell medicine Animals Cells Cultured Membrane Glycoproteins Viral Core Proteins Cell Membrane Viral tegument biology.organism_classification Cell biology Cytoplasm Vesicular stomatitis virus Insect Science Rabbits Research Article |
| Popis: | Viral assembly was studied by viewing platinum replicas of cytoplasmic and outer plasma membrane surfaces of baby hamster kidney cells infected with vesicular stomatitis virus. Replicas of the cytoplasmic surface of the basilar plasma membrane revealed nucleocapsids forming bullet-shaped tight helical coils. The apex of each viral nose cone was anchored to the membrane and was free of uncoiled nucleocapsid, whereas tortuous nucleocapsid was attached to the base of tightly coiled structures. Using immunoelectron microscopy, we identified the nucleocapsid (N) viral protein as a component of both the tight-coil and tortuous nucleocapsids, whereas the matrix (M) protein was found only on tortuous nucleocapsids. The M protein was not found on the membrane. Using immunoreagents specific for the viral glycoprotein (G protein), we found that the amount of G protein per virion varied. The G protein was consistently localized at the apex of viral buds, whereas the density of G protein on the shaft was equivalent to that in the surrounding membrane. These observations suggest that G-protein interaction with the nucleocapsid via its cytoplasmic domain may be necessary for the initiation of viral assembly. Once contact is established, nucleocapsid coiling proceeds with nose cone formation followed by formation of the helical cylinder. M protein may function to induce a nucleocapsid conformation favorable for coiling or may cross-link adjacent turns in the tight coil or both. |
| Databáze: | OpenAIRE |
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