Rapid automated disgnosis of bacteremia by impedance detection
| Autor: | Charles H. Zierdt, J D MacLowry, Robert L. Kagan, W H Schuette |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Microbiology (medical)
Bacteriological Techniques Pathology medicine.medical_specialty medicine.diagnostic_test Electric Conductivity Aerobic blood culture Biology medicine.disease Haemophilus influenzae Culture Media law.invention Sepsis Klebsiella pneumoniae law Bacteremia medicine Humans Blood culture Electrodes Filtration Research Article Biomedical engineering |
| Zdroj: | Journal of Clinical Microbiology. 5:51-57 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.5.1.51-57.1977 |
| Popis: | Lysis and filtration of blood culture specimens were combined with impedance detection of bacterial growth to facilitate the diagnosis of bacteremia. A blood lysis-filtration technique (Zierdt et al., 1976) was coupled to a simple, inexpensive automated detection system. The practical and technical aspects of the impedance detection system are discussed. This new blood culturing system was compared to a conventional system for 264 aerobic blood cultures. A 30-ml sample of the blood-broth mixture was withdrawn from the conventional aerobic blood culture bottle and processed in parallel. Excluding the isolation of the commonly recognized contaminants, the detection efficiency was 36% greater in the new system. A total of 53 blood cultures from 107 patients were positive by one or both methods. The new system detected 92% of the total number of positive cultures, compared with 56% detected by the routine method. The explanation of the differences is discussed. |
| Databáze: | OpenAIRE |
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