Biofluid metabotyping of occupationally exposed subjects to air pollution demonstrates high oxidative stress and deregulated amino acid metabolism
| Autor: | Paulraj Rajamani, Aleena Das, Ramovatar Meena, Ranjan Kumar Nanda, Surya Narayan Pradhan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Magnetic Resonance Spectroscopy Arginine Physiology Inflammation Phenylalanine Urine 010501 environmental sciences medicine.disease_cause Bioinformatics 01 natural sciences Article 03 medical and health sciences Occupational Exposure medicine Humans Amino Acids Respiratory system Lung 0105 earth and related environmental sciences Air Pollutants Glutathione Peroxidase Principal Component Analysis Multidisciplinary Superoxide Dismutase business.industry Interleukins Confounding Catalase Oxidative Stress 030104 developmental biology Spirometry Multivariate Analysis Lipid Peroxidation Occupational exposure medicine.symptom Reactive Oxygen Species business Biomarkers Oxidative stress |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Occupational exposure to air pollution induces oxidative stress and prolonged exposure increases susceptibility to cardiovascular and respiratory diseases in several working groups. Biofluid of these subjects may reflect perturbed metabolic phenotypes. In this study we carried out a comparative molecular profiling study using parallel biofluids collected from subjects (n = 85) belonging to auto rickshaw drivers (ARD), traffic cops (TC) and office workers (OW). Higher levels of oxidative stress and inflammation markers in serum of ARD subjects were observed as compared to OW and TC. Uni and multivariate analyses of metabolites identified in urine by 1H NMR revealed 11 deregulated molecules in ARD subjects and involved in phenylalanine, histidine, arginine and proline metabolism. Despite contribution of confounding factors like exposure period, dietary factors including smoking and alcohol status, our results demonstrate existence of exposure specific metabotypes in biofluids of ARD, OW and TC groups. Monitoring serum oxidative stress and inflammation markers and urine metabolites by NMR may be useful to characterize perturbed metabolic phenotypes in populations exposed to urban traffic air pollution. |
| Databáze: | OpenAIRE |
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