DHA upregulates FADS2 expression in primary cortical astrocytes exposed to vitamin A
| Autor: | E. Zgorzynska, B. Kaza, A. Walczewska, Barbara Dziedzic, D. Bewicz-Binkowska, D. Stulczewski, L. Wieteska |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Docosahexaenoic Acids Physiology medicine.drug_class FADS2 Retinoic acid Gene Expression 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Gene expression medicine Animals Retinoid Rats Wistar Receptor Vitamin A Transcription factor Cells Cultured Cerebral Cortex Messenger RNA General Medicine Rats Up-Regulation 030104 developmental biology Endocrinology chemistry Animals Newborn Docosahexaenoic acid Astrocytes 030217 neurology & neurosurgery Stearoyl-CoA Desaturase |
| Zdroj: | Scopus-Elsevier Europe PubMed Central Medical University of Lodz, Poland. PublicUM |
| ISSN: | 1802-9973 |
| Popis: | The fads2 gene encoding Δ6-desaturase, the rate-limiting enzyme of the LCPUFA biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the blood-brain barrier, may regulate the transcription of lipogenic enzymes through activation of transcription factors such as peroxisome proliferator-activated receptors (PPARs). The PPARs form the transcription complex with retinoid X receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 24-h treatment with palmitic acid (PA), α-linolenic acid (ALA) or docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed that in astrocytes not challenged with VA, PA increased fads2 gene expression and DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA levels, while DHA increased fads2 gene expression, oppositely to non-primed cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard to Δ6-desaturase protein levels following PA and DHA treatment. ALA did not have any effect on the FADS2 mRNA and protein levels in either VA-primed or non-primed astrocytes. These findings indicate that in the presence of vitamin A, DHA upregulates fads2 gene expression in astrocytes. |
| Databáze: | OpenAIRE |
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