Expression of the aging gene p66Shc is increased in peripheral blood monocytes of patients with acute coronary syndrome but not with stable coronary artery disease
| Autor: | Jan Steffel, Fabian C. Franzeck, Alexander Akhmedov, Giovanni G. Camici, Willibald Maier, Remo D. Spescha, Thomas F. Lüscher, Matthias Hasun, Felix C. Tanner, Christophe A. Wyss, Danielle Hof, Yi Shi, Arnold von Eckardstein, Francesco Cosentino |
|---|---|
| Přispěvatelé: | University of Zurich, Camici, Giovanni G |
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_treatment 030204 cardiovascular system & hematology Coronary Angiography Monocytes p66shc 10052 Institute of Physiology Coronary artery disease Pathogenesis 0302 clinical medicine Malondialdehyde 540 Chemistry oxidative stress Myocardial infarction 10038 Institute of Clinical Chemistry Whole blood Cardiac catheterization 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction acute coronary syndrome coronary artery disease Middle Aged Up-Regulation 3. Good health 10076 Center for Integrative Human Physiology Right coronary artery 10209 Clinic for Cardiology Cardiology Biomarker (medicine) Cardiology and Cardiovascular Medicine Switzerland medicine.medical_specialty Acute coronary syndrome Src Homology 2 Domain-Containing Transforming Protein 1 610 Medicine & health Real-Time Polymerase Chain Reaction 2705 Cardiology and Cardiovascular Medicine 03 medical and health sciences medicine.artery Internal medicine medicine Humans RNA Messenger 030304 developmental biology Chi-Square Distribution business.industry medicine.disease Shc Signaling Adaptor Proteins 570 Life sciences biology Lipid Peroxidation business |
| Zdroj: | Atherosclerosis |
| DOI: | 10.1016/j.atherosclerosis.2011.10.035 |
| Popis: | Objective The interplay between oxidative stress and inflammation is crucial in the pathogenesis of atherosclerosis. The adaptor protein p66Shc is implicated in atherogenesis and oxidative stress related responses in animal models of diseases. However, its role in humans remains to be defined. In this study, we hypothesized that expression of p66Shc increases in peripheral blood monocytes of patients affected by acute coronary syndromes (ACS). Methods Male subjects aged 59 ± 4 (mean ± SD) years admitted for cardiac catheterization were subdivided in three groups: (a) no local stenosis for the control group, (b) at least one stenosis ≥75% in either left, circumflex or right coronary artery for the coronary artery disease (CAD) group or (c) ST-elevation/non-ST-elevation myocardial infarction for the ACS group. Monocytes were isolated from whole blood and p66Shc RNA levels were determined by quantitative real time PCR. Results p66Shc RNA levels were increased in ACS patients as compared to CAD (p = 0.007) and controls (p = 0.0249). Furthermore, malondialdehyde (MDA) and C-reactive protein (CRP) were increased in plasma of ACS patients. Levels of MDA correlated positively to p66Shc (r = 0.376, p = 0.01). Our data demonstrate increased p66Shc levels in monocytes of ACS but not CAD patients. Conclusion This study suggests an involvement of p66Shc in the transition of a stable CAD to an ACS patient. p66Shc was associated with states of increased oxidative stress. Further work is needed to understand whether p66Shc may represent a possible pharmacological target or whether it represents an interesting novel biomarker. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|