Efficacy of a Vaccine Formula against Tuberculosis in Cattle
| Autor: | Susana Sosa Gallegos, Alejandro Nava Vargas, Leticia García Casanova, Oscar Martínez, Luis Bojorquez Narvaez, Yezenia Rubio Venegas, Feliciano Milián Suazo, Andrea M. Olvera Ramírez, Germinal Jorge Cantó Alarcón |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Tuberculosis
lcsh:Medicine Tuberculin complex mixtures Lesion Interferon-gamma Immune system medicine Animals lcsh:Science Lung Mycobacterium bovis Multidisciplinary biology business.industry Tuberculin Test lcsh:R Vaccination biology.organism_classification medicine.disease Virology Immunology BCG Vaccine lcsh:Q Cattle Lymph Lymph Nodes medicine.symptom business BCG vaccine Tuberculosis Bovine Interferon-gamma Release Tests Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 10, p e76418 (2013) |
| ISSN: | 1932-6203 |
| Popis: | "Test-and-slaughter" has been successful in industrialized countries to control and eradicate tuberculosis from cattle; however, this strategy is too expensive for developing nations, where the prevalence is especially high. Vaccination with the Calmette-Guérin (BCG) strain has been shown to protect against the development of lesions in vaccinated animals: mouse, cattle and wildlife species. In this study, the immune response and the pathology of vaccinated (BCG-prime and BCG prime-CFP-boosted) and unvaccinated (controls) calves were evaluated under experimental settings. A 10(6) CFU dose of the BCG strain was inoculated subcutaneously on the neck to two groups of ten animas each. Thirty days after vaccination, one of the vaccinated groups was boosted with an M. bovis culture filtrate protein (CFP). Three months after vaccination, the three groups of animals were challenged with 5×10(5) CFU via intranasal by aerosol with a field strain of M. bovis. The immune response was monitored throughout the study. Protection was assessed based on immune response (IFN-g release) prechallenge, presence of visible lesions in lymph nodes and lungs at slaughter, and presence of bacilli in lymph nodes and lung samples in histological analysis. Vaccinated cattle, either with the BCG alone or with BCG and boosted with CFP showed higher IFN-g response, fewer lesions, and fewer bacilli per lesion than unvaccinated controls after challenge. Animals with low levels of IFN-g postvaccine-prechallenge showed more lesions than animals with high levels. Results from this study support the argument that vaccination could be incorporated into control programs to reduce the incidence of TB in cattle in countries with high prevalence. |
| Databáze: | OpenAIRE |
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