Synthesis and preclinical evaluation of [11C]MTP38 as a novel PET ligand for phosphodiesterase 7 in the brain
| Autor: | Takehiko Matsumura, Chie Seki, Makoto Higuchi, Ming-Rong Zhang, Misae Takakuwa, Takeaki Saijo, Hajime Fukuda, Jun Maeda, Naoyuki Obokata, Takeshi Hirata, Hideki Ishii, Takafumi Minamimoto, Tatsuo Nakajima, Kazunori Kawamura, Yuji Nagai |
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| Rok vydání: | 2021 |
| Předmět: |
[11C]MTP38
0301 basic medicine Positron emission tomography Cerebellum Striatum Pharmacology Ligands 03 medical and health sciences 0302 clinical medicine In vivo Quantification medicine Radioligand Animals Tissue Distribution Radiology Nuclear Medicine and imaging Carbon Radioisotopes Cyclic Nucleotide Phosphodiesterases Type 7 medicine.diagnostic_test Chemistry Brain Binding potential Phosphodiesterase Occupancy General Medicine In vitro Rats 030104 developmental biology medicine.anatomical_structure Positron-Emission Tomography PDE7 Original Article 030217 neurology & neurosurgery |
| Zdroj: | European Journal of Nuclear Medicine and Molecular Imaging |
| ISSN: | 1619-7089 1619-7070 |
| DOI: | 10.1007/s00259-021-05269-4 |
| Popis: | Purpose Phosphodiesterase (PDE) 7 is a potential therapeutic target for neurological and inflammatory diseases, although in vivo visualization of PDE7 has not been successful. In this study, we aimed to develop [11C]MTP38 as a novel positron emission tomography (PET) ligand for PDE7. Methods [11C]MTP38 was radiosynthesized by 11C-cyanation of a bromo precursor with [11C]HCN. PET scans of rat and rhesus monkey brains and in vitro autoradiography of brain sections derived from these species were conducted with [11C]MTP38. In monkeys, dynamic PET data were analyzed with an arterial input function to calculate the total distribution volume (VT). The non-displaceable binding potential (BPND) in the striatum was also determined by a reference tissue model with cerebellar reference. Finally, striatal occupancy of PDE7 by an inhibitor was calculated in monkeys according to changes in BPND. Results [11C]MTP38 was synthesized with radiochemical purity ≥99.4% and molar activity of 38.6 ± 12.6 GBq/μmol. Autoradiography revealed high radioactivity in the striatum and its reduction by non-radiolabeled ligands, in contrast with unaltered autoradiographic signals in other regions. In vivo PET after radioligand injection to rats and monkeys demonstrated that radioactivity was rapidly distributed to the brain and intensely accumulated in the striatum relative to the cerebellum. Correspondingly, estimated VT values in the monkey striatum and cerebellum were 3.59 and 2.69 mL/cm3, respectively. The cerebellar VT value was unchanged by pretreatment with unlabeled MTP38. Striatal BPND was reduced in a dose-dependent manner after pretreatment with MTP-X, a PDE7 inhibitor. Relationships between PDE7 occupancy by MTP-X and plasma MTP-X concentration could be described by Hill’s sigmoidal function. Conclusion We have provided the first successful preclinical demonstration of in vivo PDE7 imaging with a specific PET radioligand. [11C]MTP38 is a feasible radioligand for evaluating PDE7 in the brain and is currently being applied to a first-in-human PET study. |
| Databáze: | OpenAIRE |
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