Epstein-Barr virus origin of lytic replication mediates association of replicating episomes with promyelocytic leukaemia protein nuclear bodies and replication compartments
| Autor: | Paul J. Farrell, Robert E. White, Wolfgang Amon |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Epstein-Barr Virus Infections
Herpesvirus 4 Human viruses Replication Origin Biology Promyelocytic Leukemia Protein medicine.disease_cause Origin of replication Virus Replication Replication factor C Plasmid Control of chromosome duplication Virology Cell Line Tumor medicine Humans Cell Nucleus Tumor Suppressor Proteins DNA replication Nuclear Proteins Epstein–Barr virus Neoplasm Proteins Lytic cycle DNA Viral Origin recognition complex Plasmids Transcription Factors |
| Zdroj: | The Journal of general virology. 87(Pt 5) |
| ISSN: | 0022-1317 |
| Popis: | Epstein–Barr virus (EBV) establishes a latent persistence from which it can be reactivated to undergo lytic replication. Late lytic-cycle gene expression is linked to lytic DNA replication, as it is sensitive to the same inhibitors that block lytic replication, and it has recently been shown that the viral origin of lytic replication (ori lyt) is required in cis for late-gene expression. During the lytic cycle, the viral genome forms replication compartments, which are usually adjacent to promyelocytic leukaemia protein (PML) nuclear bodies. A tetracycline repressor DNA-binding domain–enhanced green fluorescent protein fusion was used to visualize replicating plasmids carrying a tetracycline operator sequence array. ori lyt mediated the production of plasmid replication compartments that were associated with PML nuclear bodies. Plasmids carrying ori lyt and EBV itself were visualized in the same cells and replicated in similar regions of the nucleus, further supporting the validity of the plasmids for studying late-gene regulation. |
| Databáze: | OpenAIRE |
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