Molecular confinement of human amylin in lipidic nanoparticles
| Autor: | Sheila Garcia, Priscilla Tinoco, Ana Paula Gadelha, Luciana Almeida, Raquel Rennó Braga, Luiz Henrique Guerreiro, Luís Maurício T.R. Lima, Kildare Miranda, Carolina A. Braga |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
endocrine system endocrine system diseases Protein Conformation Pharmaceutical Science Amylin macromolecular substances Pharmacology Carbohydrate metabolism 010402 general chemistry 01 natural sciences Glucagon 03 medical and health sciences In vivo Humans Pancreatic hormone Liposome Chemistry Lipids In vitro 0104 chemical sciences Islet Amyloid Polypeptide Kinetics 030104 developmental biology Biochemistry Nanoparticles Homeostasis |
| Zdroj: | Journal of liposome research. 26(3) |
| ISSN: | 1532-2394 |
| Popis: | Amylin is a pancreatic hormone involved in the regulation of glucose metabolism and homeostasis. Restoration of the post-prandial and basal levels of human amylin in diabetic individuals is a key in controlling glycemia, controlling glucagon, reducing the insulin dose and increasing satiety, among other physiologic functions. Human amylin has a high propensity to aggregate. We have addressed this issue by designing a liposomal human amylin formulation. Nanoparticles of multilamellar liposomes comprising human amylin were obtained with 53% encapsulation efficiency. The in vitro kinetic release assay shows a biphasic profile. The stabilization of the lipidic nanoparticle against freeze-drying was achieved by using mannitol as a cryoprotectant, as evidenced by morphological characterization. The effectiveness of the human amylin entrapped in lipidic nanoparticles was tested by the measurement of its pharmacological effect in vivo after subcutaneous administration in mice. Collectively these results demonstrate the compatibility of human amylin with the lipidic interface as an effective pharmaceutical delivery system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|