Lipoproteins from vertebrate host blood plasma are involved in Trypanosoma cruzi epimastigote agglutination and participate in interaction with the vector insect, Rhodnius prolixus
| Autor: | Georgia C. Atella, Denise Feder, José Rodrigues Coura, Patrícia Azambuja, Helena Carla Castro, Carlos José de Carvalho Moreira, Nuccia N.T. De Cicco, Tainah Silva Galdino, Renata Bortolasse Miguel, Cicero B. Mello, Marcelo S. Gonzalez, Norman A. Ratcliffe |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chagas disease Agglutination Erythrocytes Lipoproteins Trypanosoma cruzi 030231 tropical medicine Immunology Microbiology 03 medical and health sciences 0302 clinical medicine Agglutinin Blood plasma medicine Parasite hosting Animals Humans Chagas Disease Horses Rhodnius prolixus Sheep biology Hemagglutination General Medicine biology.organism_classification Blood meal medicine.disease Insect Vectors Agglutination (biology) 030104 developmental biology Infectious Diseases Agglutinins Rhodnius Parasitology Rabbits Chickens |
| Zdroj: | Experimental parasitology. 195 |
| ISSN: | 1090-2449 |
| Popis: | Chagas disease, infecting ca. 8 million people in Central and South America, is mediated by the protozoan parasite, Trypanosoma cruzi. The parasite is transmitted by the bite of blood sucking triatomine insects, such as Rhodnius prolixus, that had previously fed on parasite-infected vertebrate blood and voided their contaminated feces and urine into the wound. The stages of the parasite life cycle in both the insect vector and human host are well-known, but determinants of infection in the insect gut are complex and enigmatic. This paper examines the possible role of the R. prolixus gut agglutinins in the parasite life cycle. The results, derived from gut extracts made from R. prolixus fed on various diets with different vertebrate blood components, and cross adsorption experiments, showed for the first time that R. prolixus has two distinct gut agglutinins originating from their vertebrate blood meal, one for T. cruzi (the parasite agglutinin, PA) and the other for the erythrocytes (the hemagglutinin, HA). Again, uniquely, the results also demonstrate that these two agglutinins are derived, respectively, from the plasma and erythrocyte components of the vertebrate blood. Subsequent experiments, examining in more detail the nature of the plasma components forming the T. cruzi PA, used fractionated extracts of the vertebrate plasma (high density lipoprotein, HDL; low density lipoprotein, LDL, and delipidated plasma) in agglutination assays. The results confirmed the identity of the PA as a high density lipoprotein (HDL) in the plasma of the vertebrate blood meal which agglutinates parasites in the R. prolixus gut. In addition, the use of single or double labeled HDL in fluorescence and confocal microscopy showed the interaction of the labeled HDL with the parasite surface and its internalization at later times. Finally, results of T. cruzi parasitization of R. prolixus, incorporating various vertebrate blood components, resulted in highly significant increases in infectivity in the presence of HDL from the 2nd day of infection, thus confirming the important role of this molecule in T. cruzi infection of R. prolixus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect