Steric protection of a modified glass surface with adsorbed polyacrylamide: Interactions with blood proteins
| Autor: | A. Schmitt, E. Pefferkorn, J.C. Voegel |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Steric effects
Chemical Phenomena Surface Properties Polyacrylamide Acrylic Resins Buffers Fibrinogen chemistry.chemical_compound Adsorption Monolayer medicine Humans chemistry.chemical_classification Chromatography Temperature Albumin Blood Proteins General Chemistry Polymer Blood proteins Chemistry chemistry Glass medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 428:17-24 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)83886-4 |
| Popis: | Glass beads chemically modified with aluminol (AlOH) groups were coated with a monolayer of high-molecular-mass polyacrylamide at different temperatures and exposed to 125I-labelled albumin and fibrinogen solutions for 1 h. No adsorption of albumin was observed, whereas fibrinogen adsorption increased as a function of temperature. These observations were interpreted in terms of the density and stability of the adsorbed polymer layer. At 25°C, where fibrinogen adsorption was already important, it was shown, using 3H-labelled polyacrylamide, that fibrinogen partly displaced the polymer from its adsorption sites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|