Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs
| Autor: | Dong Sun, Rodger Main, Leslie Bower, Gregory W. Stevenson, B. L. Wilberts, Chong Wang, Phillip C. Gauger, Drew R. Magstadt, Paulo Arruda, Angela Pillatzki, Hai Hoang, Eric R. Burrough, Kyoung-Jin Yoon, Jianqiang Zhang, Karen M. Harmon, J. Brodie, Mahesh Bhandari, Darin M. Madson |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Diarrhea
Swine viruses Weaning Real-Time Polymerase Chain Reaction Microbiology Enteritis Feces Intestine Small Animals Medicine Viral shedding DNA Primers Swine Diseases General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction business.industry Porcine epidemic diarrhea virus Body Weight Temperature General Medicine biology.organism_classification medicine.disease Immunohistochemistry Virology Virus Shedding Staining Enterocytes Linear Models biology.protein medicine.symptom Antibody Coronavirus Infections business |
| Zdroj: | Veterinary Microbiology. 174:60-68 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2014.09.002 |
| Popis: | Porcine epidemic diarrhea virus (PEDV) is associated with clinical diarrhea in naive swine of all ages. This report describes timing of antibody generation and disease progression following infection with a US PEDV isolate by assessing fecal viral shedding, morphometric analysis of intestinal lesions, and magnitude of immunohistochemical staining. Sixty-three, 3-week-old pigs were randomly allocated into control (n=27) and challenged (n=36) groups. Challenged pigs were administered 1 mL of 1 × 10(3) PFU/mL of US/Iowa/18984/2013 PEDV isolate by oro-gastric gavage. Three control and four challenged pigs were necropsied on days post-inoculation (dpi) 1, 2, 3, 4, 7, and weekly thereafter, until study termination on dpi 35. Clinical disease, fecal shedding, body weight, and temperature were monitored during the study period. Diarrhea was observed in challenged pigs beginning for some on dpi 2, affecting a majority of pigs by dpi 6 and subsiding by dpi 10. Average daily gain was significantly lower (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect