Hemizona assay (HZA) demonstrates effects of characterized mouse antihuman sperm antibodies on sperm zona binding*
| Autor: | Gary D. Hodgen, David L. Fulgham, Mary C. Mahony, Nancy J. Alexander, Charles C. Coddington, D. Johnson |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system medicine.drug_class Urology Semen Monoclonal antibody Mice Endocrinology Antigen medicine Animals Humans Antigens Acrosome Zona pellucida Zona Pellucida reproductive and urinary physiology Sperm-Ovum Interactions biology urogenital system Antibodies Monoclonal General Medicine Spermatozoa Molecular biology Sperm medicine.anatomical_structure Immunoglobulin M Immunoglobulin G biology.protein Gamete Female Antibody |
| Zdroj: | Andrologia. 24:271-277 |
| ISSN: | 1439-0272 0303-4569 |
| Popis: | Summary. Characterized antihuman sperm monoclonal antibodies from mice were evaluated using the hemizona assay (HZA) to determine whether sperm: zona binding was effected. The seven monoclonal antibodies were characterized using human sperm in agglutination, immobilization, and penetration assays. Semen was provided by four fertile men and used in the HZA to determine if the presence of a monoclonal antibody would affect tight binding of the sperm to the zona pellucida. Pre-incubation of MA-14 for 1 h with the sperm induced a 33–54% reduction of the number of tightly bound sperm. This antibody reacts to an antigen located on the acrosome and midpiece. Experiments in which there was no pre-incubation of the antibody with sperm, resulted in no significant reduction in the number of sperm bound in the HZA. These findings suggest that an anti-human sperm antibody produced in mice can modulate sperm: zona binding. Reduction in zona binding could indicate a cause of immune-related infertility and this test may be useful in selecting an antigen for contraceptive vaccine development. |
| Databáze: | OpenAIRE |
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