Cytotoxicity of functionalized carbon nanotubes in J774A macrophages
| Autor: | Carmen González Horta, Patricia Talamás-Rohana, Alfredo Aguilar-Elguezabal, Erasmo Orrantia-Borunda, Blanca Sánchez-Ramírez, Silvia Lorena Montes-Fonseca |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Cell Survival Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Apoptosis Cell Line Entamoeba histolytica chemistry.chemical_compound Mice Materials Testing Cytotoxic T cell Animals General Materials Science Viability assay Fluorescein Cytotoxicity biology Dose-Response Relationship Drug Nanotubes Carbon Macrophages biology.organism_classification chemistry Biochemistry Cell culture Toxicity Molecular Medicine |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 8(6) |
| ISSN: | 1549-9642 |
| Popis: | Cytotoxicity of carbon nanotubes (CNTs) is a prime concern for its use as antigen carriers. Here we evaluated the cytotoxic effect of unpurified (UP-CNTs), purified (P-CNTs), fluorescein isothiocyanate-functionalized (FITC-CNTs), and Entamoeba histolytica 220-kDa lectin-functionalized CNTs (L220-CNTs) in J774A macrophage (MOs) cell line. Cell viability and apoptosis were analyzed by MTT and TUNEL assays, respectively. Cyclooxygenase-2 (COX-2) was analyzed by reverse transcription-polymerase chain reaction. Cytotoxicity at 6.0 mg/L was higher with UP-CNTs > P-CNTs > FITC-CNTs, showing a decrease in cell viability and an increase in apoptosis. In contrast, MOs interacted with L220-CNTs showed an increase in cell viability without signs of apoptosis. Although UP-CNTs and P-CNTs exhibited COX-2 induction with 6.0 mg/L, functionalized CNTs were able to induce COX-2 at concentrations as low as 0.06 mg/L. These results suggest that functionalization decreases toxicity, and that L220-CNTs may be an excellent candidate for the production of a nanovaccine against amebiasis. |
| Databáze: | OpenAIRE |
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