Set of Highly Stable Amine- and Carboxylate-Terminated Dendronized Au Nanoparticles with Dense Coating and Nontoxic Mixed-Dendronized Form
| Autor: | Maureen A. Kane, Arunendra Saha Ray, Marie-Christine Daniel, William Ghann, Yewon J Pak, Lance T. Dockery, Wenjing Li, Brian Szychowski, Phoebe S. Tsoi, Peter W. Swaan |
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| Rok vydání: | 2019 |
| Předmět: |
Dendrimers
Metal Nanoparticles Nanoparticle 02 engineering and technology Polypropylenes 010402 general chemistry 01 natural sciences Article Propyleneimine chemistry.chemical_compound Dendrimer Monolayer Electrochemistry Humans General Materials Science Carboxylate Spectroscopy Thioctic Acid Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Combinatorial chemistry 0104 chemical sciences chemistry Drug delivery MCF-7 Cells Surface modification Gold Propionates Nanocarriers 0210 nano-technology |
| Zdroj: | Langmuir. 35:3391-3403 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | The synthesis of a novel poly(propyleneimine) (PPI) dendron in gram scale as well as its use in the formation of a highly stable, dendronized gold nanoparticle (AuNP)-based drug delivery platform is described herein. The AuNP-based platform is composed of three complementary parts: (i) a 15 nm AuNP core, (ii) a heterofunctional thioctic acid-terminated tetraethylene glycol spacer, and (iii) a third-generation PPI dendron with a unique protonation profile and diverse end-group functionalization that allows for further derivatization. The prepared dendronized AuNPs are able to withstand several rounds of lyophilization cycles with no sign of aggregation, are stable in phosphate-buffered saline and Hanks’ buffer as well as in serum, and are resistant to degradation by glutathione exchange reactions. This nanocarrier platform displays a dense coating, with >1400 dendrons/AuNPs, which will enable very high payload. Furthermore, while amine-terminated AuNPs expectedly showed cytotoxicity against the MCF-7 breast cancer cell line from a NP concentration of 1 nM, the mixed monolayer AuNPs (coated with 40/60 amine/carboxylate dendrons) interestingly did not exhibit any sign of toxicity at concentrations as high as 15 nM, similar to the carboxylate-terminated AuNPs. The described dendronized AuNPs address the current practical need for a stable NP-based drug delivery platform which is scalable and easily conjugable, has long-term stability in solution, and can be conveniently formulated as a powder and redispersed in desired buffer or serum. |
| Databáze: | OpenAIRE |
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