CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
| Autor: | Zhenya Zhai, Hui-Chao Yan, Qian-yun Xi, Wu-tai Guan, Gao-feng Xu, Chun-Qi Gao, Song-bo Wang, Xiu-Qi Wang, Xiang-guang Li |
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| Rok vydání: | 2016 |
| Předmět: |
pig
Male 0301 basic medicine Amino Acid Transport System y+ Swine Cyclin B Cell Line 03 medical and health sciences Intestinal mucosa Cyclin D2 Cyclin-dependent kinase Cyclins Animals CDX2 Transcription Factor Amino Acid Sequence Intestinal Mucosa Promoter Regions Genetic CDX2 Sequence Homology Amino Acid biology Epithelial Cells Cell cycle Intestinal epithelium Molecular biology digestive system diseases Cyclin-Dependent Kinases cell proliferation 030104 developmental biology Gene Expression Regulation Oncology embryonic structures biology.protein solute carrier family 7 member 7 intestinal epithelial cells Female Cyclin A2 Research Paper Protein Binding |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.8894 |
| Popis: | // Xiang-guang Li 1 , Gao-feng Xu 1 , Zhen-ya Zhai 1 , Chun-qi Gao 1 , Hui-chao Yan 1 , Qian-yun Xi 1 , Wu-tai Guan 1 , Song-bo Wang 1 , Xiu-qi Wang 1 1 College of Animal Science, South China Agricultural University/National Engineering Research Center for Breeding Swine Industry, Guangzhou 510642, China Correspondence to: Xiu-qi Wang, email: xqwang@scau.edu.cn Keywords: CDX2, cell proliferation, intestinal epithelial cells, solute carrier family 7 member 7, pig Received: January 11, 2016 Accepted: March 31, 2016 Published: April 21, 2016 ABSTRACT Nutrient absorption mediated by nutrient transporters expressed in the intestinal epithelium supplies substrates to support intestinal processes, including epithelial cell proliferation. We evaluated the role of Caudal type homeobox 2 (CDX2), an intestine-specific transcription factor, in the proliferation of pig intestinal epithelial cells (IPEC-1) and searched for novel intestinal nutrient transporter genes activated by CDX2. Our cloned pig CDX2 cDNA contains a “homeobox” DNA binding motif, suggesting it is a transcriptional activator. CDX2 overexpression in IPEC-1 cells increased cell proliferation, the percentage of cells in S/G2 phase, and the abundance of transcripts of the cell cycle-related genes Cyclin A2 ; Cyclin B ; Cyclin D2 ; proliferating cell nuclear antigen ; and cell cycle cyclin-dependent kinases 1, 2 and 4 , as well as the predicted CDX2 target genes SLC1A1, SLC5A1 and SLC7A7 . In addition, luciferase reporter and chromatin immunoprecipitation assays revealed that CDX2 binds directly to the SLC7A7 promoter. This is the first report of CDX2 function in pig intestinal epithelial cells and identifies SLC7A7 as a novel CDX2 target gene. Our findings show that nutrient transporters are activated during CDX2-induced proliferation of normal intestinal epithelial cells. |
| Databáze: | OpenAIRE |
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