Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. and in humans with Chagas disease associated with oral transmission
| Autor: | Arlei Marcili, Flávia Maia da Silva, Sebastião Aldo da Silva Valente, Vera da Costa Valente, José Rodrigues Coura, Erney P. Camargo, Michael A. Miles, R. D. Naiff, Marta Maria Geraldes Teixeira, Marta Campaner, Angela Cristina Verissimo Junqueira, Ana Yecê das Neves Pinto |
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| Rok vydání: | 2009 |
| Předmět: |
Primates
Chagas disease Genotype Trypanosoma cruzi Rhodnius Zoology Species Specificity Phylogenetics parasitic diseases medicine Animals Humans Chagas Disease Phylogeny Polymorphism Genetic biology Cytochrome b Monkey Diseases Kinetoplastida Cytochromes b DNA Protozoan biology.organism_classification medicine.disease Insect Vectors Random Amplified Polymorphic DNA Technique Infectious Diseases Reduviidae Cebidae Aotidae Parasitology Saguinus Trypanosomiasis Brazil |
| Zdroj: | International Journal for Parasitology. 39:615-623 |
| ISSN: | 0020-7519 |
| Popis: | In this study, we provide phylogenetic and biogeographic evidence that the Trypanosoma cruzi lineages T. cruzi I (TCI) and T. cruzi IIa (TCIIa) circulate amongst non-human primates in Brazilian Amazonia, and are transmitted by Rhodnius species in overlapping arboreal transmission cycles, sporadically infecting humans. TCI presented higher prevalence rates, and no lineages other than TCI and TCIIa were found in this study in wild monkeys and Rhodnius from the Amazonian region. We characterised TCI and TCIIa from wild primates (16 TCI and five TCIIa), Rhodnius spp. (13 TCI and nine TCIIa), and humans with Chagas disease associated with oral transmission (14 TCI and five TCIIa) in Brazilian Amazonia. To our knowledge, TCIIa had not been associated with wild monkeys until now. Polymorphisms of ssrDNA, cytochrome b gene sequences and randomly amplified polymorphic DNA (RAPD) patterns clearly separated TCIIa from TCIIb-e and TCI lineages, and disclosed small intra-lineage polymorphisms amongst isolates from Amazonia. These data are important in understanding the complexity of the transmission cycles, genetic structure, and evolutionary history of T. cruzi populations circulating in Amazonia, and they contribute to both the unravelling of human infection routes and the pathological peculiarities of Chagas disease in this region. |
| Databáze: | OpenAIRE |
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