Arginine vasopressin-Venus reporter mice as a tool for studying magnocellular arginine vasopressin neurons
| Autor: | Takeshi Onoue, Shintaro Iwama, Tomoko Kobayashi, Yoshihiro Ito, Daisuke Hagiwara, Yohei Kawaguchi, Hidetaka Suga, Tetsuro Tsumura, Takashi Miyata, Hiroshi Arima, Yuichi Hodai, Hiroshi Takagi, Yoshinori Azuma, Masayoshi Tochiya, Ryoichi Banno, Mariko Sugiyama |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system Vasopressin medicine.medical_specialty Arginine Physiology 030209 endocrinology & metabolism Biochemistry Fluorescence Mice 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Endocrinology Bacterial Proteins Downregulation and upregulation Genes Reporter Posterior pituitary Internal medicine medicine Animals Neurons Mice Inbred ICR Forskolin urogenital system Arginine Vasopressin Mice Inbred C57BL Luminescent Proteins medicine.anatomical_structure nervous system chemistry Hypothalamus Magnocellular cell Female hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Antidiuretic |
| Zdroj: | Peptides. 139:170517 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2021.170517 |
| Popis: | Arginine vasopressin (AVP) synthesized in the magnocellular neurons of the hypothalamus is transported through their axons and released from the posterior pituitary into the systemic circulation to act as an antidiuretic hormone. AVP synthesis and release are precisely regulated by changes in plasma osmolality. Magnocellular AVP neurons receive innervation from osmosensory and sodium-sensing neurons, but previous studies showed that AVP neurons per se are osmosensitive as well. In the current study, we made AVP-Venus reporter mice and showed that Venus was expressed exclusively in AVP neurons and was upregulated under water deprivation. In hypothalamic organotypic cultures from the AVP-Venus mice, Venus-labeled AVP neurons in the supraoptic and paraventricular nuclei survived for 1 month, and Venus expression was upregulated by forskolin. Furthermore, in dissociated Venus-labeled magnocellular neurons, treatment with NaCl, but not with mannitol, decreased Venus fluorescence in the soma of the AVP neurons. Thus, Venus expression in AVP-Venus transgenic mice, as well as in primary cultures, faithfully showed the properties of intrinsic AVP expression. These findings indicate that AVP-Venus mice as well as the primary hypothalamic cultures could be useful for studying magnocellular AVP neurons. |
| Databáze: | OpenAIRE |
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