LC/MS/MS analyses of open-flow microperfusion samples quantify eicosanoids in a rat model of skin inflammation
| Autor: | Natalie Bordag, Bernadette Reiter, Thomas Birngruber, Cornelia Pipper, Manfred Bodenlenz, Peter Florian, Anita Eberl, Frank Sinner, Kyriakos D. Economides |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Microdialysis Leukotriene B4 Anti-Inflammatory Agents Prostaglandin Inflammation QD415-436 030204 cardiovascular system & hematology Pharmacology Skin Diseases Biochemistry prostaglandins Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Tandem Mass Spectrometry Interstitial fluid leukotrienes Psoriasis medicine liquid chromatography Animals solid-phase extraction Glucocorticoids Research Articles Chromatography High Pressure Liquid Skin Solid Phase Extraction psoriasis Cell Biology medicine.disease Rats Thromboxane B2 Disease Models Animal 030104 developmental biology chemistry Eicosanoid Eicosanoids lipids (amino acids peptides and proteins) medicine.symptom |
| Zdroj: | Journal of Lipid Research, Vol 60, Iss 4, Pp 758-766 (2019) |
| ISSN: | 0022-2275 |
| Popis: | Eicosanoids are lipid-mediator molecules with key roles in inflammatory skin diseases, such as psoriasis. Eicosanoids are released close to the source of inflammation, where they elicit local pleiotropic effects and dysregulations. Monitoring inflammatory mediators directly in skin lesions could provide new insights and therapeutic possibilities. Here, we analyzed dermal interstitial fluid samples obtained by dermal open-flow microperfusion in a rat model of skin inflammation. We developed a solid-phase extraction ultra-HPLC/MS/MS method to reliably and precisely analyze small-volume samples and quantified 11 eicosanoids [thromboxane B(2), prostaglandin (PG) E(2), PGD(2), PGF(2α), leukotriene B(4), 15-HETE, 12-HETE, 5-HETE, 12-hydroxyeicosapentaenoic acid, 13-HODE, and 17-hydroxydocosahexaenoic acid]. Our method achieved a median intraday precision of approximately 5% and interday precision of approximately 8%. All calibration curves showed excellent linearity between 0.01 and 50 ng/ml (R(2) > 0.980). In the rat model, eicosanoids were significantly increased in imiquimod-treated inflamed skin sites compared with untreated control sites. Oral treatment with an anti-inflammatory glucocorticoid decreased eicosanoid concentrations. These results show that a combination of tissue-specific sampling with LC/MS analytics is well suited for analyzing small sample volumes from minimally invasive sampling methods such as open-flow microperfusion or microdialysis to study local inflammation and the effect of treatments in skin diseases. |
| Databáze: | OpenAIRE |
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