Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study
| Autor: | Mohammad Froughreyhani, Mohammad Hosein-Soroush, Amin Salem-Milani, Aysan Mozafari |
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| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Serial dilution Root canal medicine.medical_treatment Enterococcus faecalis Operative Dentistry and Endodontics 0404 agricultural biotechnology food medicine Agar General Dentistry Saline Electronic apex locator Chromatography biology Chemistry Research Chlorhexidine Direct current 04 agricultural and veterinary sciences biology.organism_classification CIENCIAS MÉDICAS [UNESCO] 040401 food science medicine.anatomical_structure UNESCO::CIENCIAS MÉDICAS medicine.drug |
| Zdroj: | Foroughreyhani, Mohammad ; Salemmilani, Amin ; Mozafari, Aysan ; Soroush-Barhaghi, Mohammadhosien. Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study. En: Journal of Clinical and Experimental Dentistry, 10 12 2018: 1223-1229 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname Journal of Clinical and Experimental Dentistry |
| Popis: | Background This in vitro study was mainly aimed to evaluate the effect of high-frequency alternating currents (AC) applied by an electronic apex locator (EAL) on the antibacterial properties of chlorhexidine (CHX) on E. faecalis biofilm. Material and Methods The root canals of 120 extracted human single-rooted teeth were prepared using Gates-Glidden drills and hand K-files. After contaminating the root canals with E. faecalis, they were incubated for 60 days. Then, the teeth were randomly divided into six experimental groups (n=20). Group 1, 2% CHX; group 2, normal saline (NS) with direct current (DC); group 3, normal saline (NS) with high-frequency alternating current (AC); group 4, 2% CHX with DC; group 5, 2% CHX with AC; group 6, control (normal saline). The samples were collected from the root canal walls of 16 teeth in each group and 1:10 serial dilutions were prepared and added to Muller-Hinton agar (MHA) plates and incubated at 37°C for 48 h. The longitudinal sections of the other 4 teeth used to observe under a scanning electron microscope (SEM). A classic colony counting technique was used for counting the vital E. faecalis bacteria in MHA. Two-way ANOVA was used for statistical analysis of the data. The level of significance was set at P |
| Databáze: | OpenAIRE |
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