Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders
| Autor: | T.M. Gliozzi, Luisa Zaniboni, Silvia Cerolini, Nicolaia Iaffaldano |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Motility Semen Biology Dimethylacetamide Cryopreservation law.invention Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents law Pellet Animals plasma membrane integrity urogenital system business.industry Extender Cell Membrane 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine DNA 040201 dairy & animal science Spermatozoa Staining Biotechnology Chickens DNA integrity pellet cryopreservation spermatozoa 030104 developmental biology chemistry Animal Science and Zoology business Food Science Semen Preservation |
| Zdroj: | British poultry science. 58(5) |
| ISSN: | 1466-1799 |
| Popis: | 1. Aim of this study was the development of an optimised cryopreservation pellet procedure for chicken semen and the assessment of DNA and membrane integrity in frozen/thawed spermatozoa in a Hubbard F15 meat type selected strain.2. The following semen processing conditions were studied: spermatozoa working concentration (SWC), 1.5 vs 2 × 109 cells/ml in pre-freezing extender; equilibration of diluted semen at 5°C, 20 vs 40 min; dimethylacetamide concentration, 6% vs 9%; dimethylacetamide equilibration time at 5°C, 1 vs 30 min; thawing at 60°C for 10 vs 50°C for 30 sec. Spermatozoa viability (EtBr exclusion procedure – stress test), mobility (Accudenz® swim-down test) and subjective motility were assessed in fresh and frozen-thawed semen.3. The lower SWC (1.5 × 109 cells/ml) and the higher dimethylacetamide concentration (9%) had positive significant effects on the recovery rate of motile (22% vs 16%) and viable spermatozoa (39 vs 34%), respectively.4.Membrane (SYBR14-PI staining) and DNA integrit... |
| Databáze: | OpenAIRE |
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