Continuous-Flow Production of Injectable Liposomes via a Microfluidic Approach
| Autor: | Alessandra Zizzari, Luigi Carbone, Monica Bianco, Giuseppe Maruccio, Elisabetta Perrone, Filippo Rendina, Valentina Arima, Francesco Amato |
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| Přispěvatelé: | Zizzari, Alessandra, Bianco, Monica, Carbone, Luigi, Perrone, MARIA ELISABETTA, Amato, Francesco, Maruccio, Giuseppe, Rendina, Filippo, Arima, Valentina |
| Rok vydání: | 2017 |
| Předmět: |
liposomes
Materials science Dispersity Microfluidics microfluidics 02 engineering and technology 010402 general chemistry lcsh:Technology 01 natural sciences Article General Materials Science Solubility lcsh:Microscopy lcsh:QC120-168.85 Liposome lcsh:QH201-278.5 lcsh:T 021001 nanoscience & nanotechnology microreactors 0104 chemical sciences Microreactor Membrane Microfluidic Chemical engineering lcsh:TA1-2040 Hydrodynamic focusing lcsh:Descriptive and experimental mechanics Materials Science (all) lcsh:Electrical engineering. Electronics. Nuclear engineering Particle size lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 |
| Zdroj: | Materials Materials; Volume 10; Issue 12; Pages: 1411 Materials, Vol 10, Iss 12, p 1411 (2017) |
| ISSN: | 1996-1944 |
| Popis: | Injectable liposomes are characterized by a suitable size and unique lipid mixtures, which require time-consuming and nonstraightforward production processes. The complexity of the manufacturing methods may affect liposome solubility, the phase transition temperatures of the membranes, the average particle size, and the associated particle size distribution, with a possible impact on the drug encapsulation and release. By leveraging the precise steady-state control over the mixing of miscible liquids and a highly efficient heat transfer, microfluidic technology has proved to be an effective and direct methodology to produce liposomes. This approach results particularly efficient in reducing the number of the sizing steps, when compared to standard industrial methods. Here, Microfluidic Hydrodynamic Focusing chips were produced and used to form liposomes upon tuning experimental parameters such as lipids concentration and Flow-Rate-Ratios (FRRs). Although modelling evidenced the dependence of the laminar flow on the geometric constraints and the FRR conditions, for the specific formulation investigated in this study, the lipids concentration was identified as the primary factor influencing the size of the liposomes and their polydispersity index. This was attributed to a predominance of the bending elasticity modulus over the vesiculation index in the lipid mixture used. Eventually, liposomes of injectable size were produced using microfluidic one-pot synthesis in continuous flow. |
| Databáze: | OpenAIRE |
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