β-galactosidase ω-complementation with a small cyanogen bromide peptide
| Autor: | Wlodzimierz Mandecki, Audree V. Fowler, Joseph K. Welply, Irving Zabin |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Mutant
Biophysics lac operon Peptide medicine.disease_cause Biochemistry chemistry.chemical_compound Species Specificity Escherichia coli medicine Cyanogen Bromide Molecular Biology chemistry.chemical_classification Mutation Chemistry Genetic Complementation Test Tryptic peptide Cell Biology beta-Galactosidase Molecular biology Peptide Fragments Galactosidases Complementation Kinetics Cyanogen bromide |
| Zdroj: | Biochemical and Biophysical Research Communications. 93:223-227 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(80)80269-5 |
| Popis: | Summary Complementation has been found to occur between extracts of lacZ X90, an operator distal nonsense mutant of E. coli , and CNBr24,the 32-residue carboxyl-terminal cyanogen bromide peptide from β-galactosidase. The degree of complementation is less than between X90 protein and a large carboxyl terminal fragment from lacZ B9 but the times required are similar. Other peptides including the 10-residue carboxyl terminal tryptic peptide are ineffective in complementation. |
| Databáze: | OpenAIRE |
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