Molecular cloning and sequence analysis of the phosphoprotein, nucleocapsid protein, matrix protein and 22K (M2) protein of the ovine respiratory syncytial virus
| Autor: | L. N. D. Potgieter, H. Alansari |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Genes Viral Sequence analysis viruses Molecular Sequence Data Molecular cloning Biology Viral Matrix Proteins Viral Proteins Capsid Viral Envelope Proteins Sequence Homology Nucleic Acid Virology Animals Humans Amino Acid Sequence Cloning Molecular Conserved Sequence Viral Structural Proteins Cloning chemistry.chemical_classification HN Protein Sheep Viral matrix protein Base Sequence Sequence Homology Amino Acid Strain (chemistry) Structural gene Sequence Analysis DNA Respiratory Syncytial Viruses chemistry Phosphoprotein Cattle Glycoprotein |
| Zdroj: | Journal of General Virology. 75:3597-3601 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-75-12-3597 |
| Popis: | Respiratory syncytial viruses (RSVs) cause serious respiratory tract disease in infants and children worldwide and similar disease in calves. Strains have been isolated from other ruminant animals such as sheep and goats, but these viruses have not been characterized at the molecular level. In this study, we report the cloning and sequencing of four structural genes coding for the phosphoprotein, nucleocapsid (N) protein, matrix (M) protein and 22K protein of an ovine RSV strain. Comparisons of these sequences with those of bovine and human RSV show that the M and N proteins are the most conserved between ruminant RSV strains and the N protein is the most conserved protein between human and ruminant RSV strains. The attachment G glycoprotein and the small hydrophobic protein are the most divergent proteins among human and ruminant RSV subgroups. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|