Free radicals run in lizard families: a mitochondrial uncoupling phenomenon or not?
| Autor: | Frédéric Bouillaud, François Criscuolo |
|---|---|
| Přispěvatelé: | Département Ecologie, Physiologie et Ethologie (DEPE-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Biologie des Transporteurs Mitochondriaux et Métabolisme (BIOTRAM), Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Aging
Carbonyl Cyanide m-Chlorophenyl Hydrazone Free Radicals MESH: Mitochondria Radical MESH: Lizards Biology medicine.disease_cause Invited Reply 03 medical and health sciences 0302 clinical medicine Species Specificity MESH: Membrane Potential Mitochondrial biology.animal medicine Animals MESH: Animals ComputingMilieux_MISCELLANEOUS 030304 developmental biology Free-radical theory of aging Fluorescent Dyes chemistry.chemical_classification Membrane Potential Mitochondrial 0303 health sciences Reactive oxygen species MESH: Oxidative Stress Lizard [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Comment Hydrazones MESH: Reactive Oxygen Species Lizards Flow Cytometry Agricultural and Biological Sciences (miscellaneous) Biological Evolution Mitochondria Oxidative Stress chemistry Biochemistry Ageing [SDE]Environmental Sciences General Agricultural and Biological Sciences Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress MESH: Hydrazones |
| Zdroj: | Biology Letters Biology Letters, Royal Society, The, 2009, 5 (3), pp.343-344. ⟨10.1098/rsbl.2009.0047⟩ |
| ISSN: | 1744-9561 1471-2954 |
| DOI: | 10.1098/rsbl.2009.0047⟩ |
| Popis: | In the ageing individual, the production of reactive oxygen species (ROS) accelerates with cell senescence. Depending on the heritability of the underlying processes that determine net ROS levels, this may influence ageing per se and its evolutionary direction and rate of change. In order to understand the inheritance and evolution of net ROS levels in free-ranging lizards, we used flow cytometry together with ROS-sensitive fluorogenic probes to measure ROS in lizard blood cells. We measured basal levels of (i) non-specific ROS (superoxide, singlet oxygen, H2O2 and peroxynitrite), (ii) superoxide specifically and (iii) superoxide after CCCP treatment, which elevated ROS production in the mitochondria. The cumulative level of non-specific ROS was higher in adults than juveniles and superoxide level showed high heritability and variability among families. We suggest that the evolution of ROS dynamics may be ROS species specific and perhaps depend on the relative degree of uni- or biparental inheritance of ROS main regulatory pathways. |
| Databáze: | OpenAIRE |
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