Enzyme-Powered Liquid Metal Nanobots Endowed with Multiple Biomedical Functions
| Autor: | Jing Hu, Xi Pan, Samuel Sanchez, Xiaohui Yan, Dandan Xu, Xing Ma |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Liquid metal
Materials science Urease enzyme Microfluidics Metal Nanoparticles General Physics and Astronomy Nanoparticle Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Mice Animals General Materials Science Drug Carriers Photothermal effect General Engineering Phototherapy Photothermal therapy 021001 nanoscience & nanotechnology Urease 0104 chemical sciences Positive chemotaxis Nanoparticles 0210 nano-technology Drug carrier |
| Zdroj: | ACS Nano. 15:11543-11554 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.1c01573 |
| Popis: | Catalytically powered micro/nanobots (MNBs) can perform active movement by harnessing energy fromiin situ/ichemical reactions and show tremendous potential in biomedical applications. However, the development of imageable MNBs that are driven by bioavailable fuels and possess multiple therapeutic functions remains challenging. To resolve such issues, we herein propose enzyme (urease) powered liquid metal (LM) nanobots that are naturally of multiple therapeutic functions and imaging signals. The main body of the nanobot is composed of a biocompatible LM nanoparticle encapsulated by polydopamine (PDA). Urease enzyme needed for the powering and desired drug molecules,ie/i.ig/i., cefixime trihydrate antibiotic, are grafted on external surfaces of the PDA shell. Such a chemical composition endows the nanobots with dual-mode ultrasonic (US) and photoacoustic (PA) imaging signals and favorable photothermal effect. These LM nanobots exhibit positive chemotaxis and therefore can be collectively guided along a concentration gradient of urea for targeted transportation. When exposed to NIR light, the LM nanobots would deform and complete the function change from active drug carriers to photothermal reagents, to achieve synergetic antibacterial treatment by both photothermal and chemotherapeutic effects. The US and PA properties of the LM nanoparticle can be used to not only track and monitor the active movement of the nanobots in a microfluidic vessel model but also visualize their dynamics in the bladder of a living mouseiin vivo/i. To conclude, the LM nanobots demonstrated herein represent a proof-of-concept therapeutic nanosystem with multiple biomedical functionalities, providing a feasible tool for preclinical studies and clinical trials of MNB-based imaging-guided therapy. |
| Databáze: | OpenAIRE |
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