Structure and promoter activity of a stress and developmentally regulated polyubiquitin-encoding gene of Nicotiana tabacum
| Autor: | Bertrand Plesse, Pascal Genschik, Jacqueline Marbach, Jacqueline Fleck, M. Uze, M. Feuerman |
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| Rok vydání: | 1994 |
| Předmět: |
Nicotiana tabacum
DNA Mutational Analysis Molecular Sequence Data Genes Plant Primer extension Biopolymers Start codon Complementary DNA Tobacco Gene expression Genetics Genomic library Amino Acid Sequence Polyubiquitin Promoter Regions Genetic Ubiquitins Gene Base Sequence biology Protoplasts Intron Gene Expression Regulation Developmental General Medicine biology.organism_classification Molecular biology Plants Toxic Protein Biosynthesis |
| Zdroj: | Gene. 148:195-202 |
| ISSN: | 0378-1119 |
| Popis: | A polyubiquitin-encoding gene was identified from a Nicotiana tabacum genomic library using a specific probe spanning the 3' untranslated region of the corresponding cDNA. The gene, Ubi.U4, is expressed in various amounts in the whole plant, except in just-fully-expanded leaves. Genomic blots indicate that it originates from N. tomentosiformis. Sequence analyses reveal that the gene consists of four ubiquitin monomers extended by a fifth truncated subunit. It is disrupted by a single 457-bp intron in close proximity to the start codon of translation. Primer extension experiments localized the transcription start point (tsp). Transient gene expression in N. tabacum protoplasts indicates that the deletion of the intron has no significant influence on gene expression. Mutagenesis on putative cis-regulatory elements indicates at least three important motifs in the proxiroal promoter: an ‘ACGT’ core element, an A+T-rich sequence and a less clearly defined cis-element located between bp −162 and −113. |
| Databáze: | OpenAIRE |
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